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Amino Acids

, Volume 15, Issue 1–2, pp 179–186 | Cite as

Effects ofin vivo taurine depletion on induced- chemiluminescence production in macrophages isolated from rat lungs

  • X. Zhang
  • J. B. Lombardini
Full Papers

Summary

Alveolar macrophages isolated by pulmonary lavage from partially taurine-depleted ratsdemonstrated increased (2–2.6 fold) chemiluminescence due to the extracellular reaction between exogenous zymosan and various reactive forms of oxygen compared to macrophages isolated from control animals. Partial taurine depletion was achieved by adding 3% ß-alanine to the drinking water of the rats for 5 weeks prior to harvesting the macrophages. Superoxide dismutase activity was not increased in the lung tissue of the taurine-depleted rats. These data suggest that taurine has antioxidant properties and that taurine depletion is potentially deleterious to alveolar macrophages and pulmonary tissue.

Keywords

Amino acids Taurine Superoxide anion Chemiluminescence Superoxide dismutase Macrophage 

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References

  1. Banks MA, Porter DW, Martin WG, Castranova V (1990) Effects of in vitro ozone exposure on peroxidative damage, membrane leakage, and taurine content of rat alveolar macrophages. Toxicol Appl Pharmacol 105: 55–65Google Scholar
  2. Banks MA, Porter DW, Martin WG, Castranova V (1991) Ozone-induced lipid peroxidation and membrane leakage in isolated rat alveolar macrophages: protective effects of taurine. J Nutr Biochem 2: 308–313Google Scholar
  3. Banks MA, Porter DW, Martin WG, Castranova V (1992) Taurine protects against oxidant injury to rat alveolar pneumocytes. In: Lombardini JB, Schaffer SW, Azuma J (eds) Taurine: nutritional value and mechanisms of action. Plenum Press, New York, pp 341–354Google Scholar
  4. Castranova V, Bowman L, Reasor MJ, Miles PR (1980) Effects of heavy metal ions on selected oxidative metabolic processes in rat alveolar macrophages. Tox Appl Pharmacol 53: 14–23Google Scholar
  5. Gaull GG, Pasantes-Morales H, Wright CE (1985) Taurine in human nutrition: overview. In: Ota SS, Ahtel L, Kontro P, Paasonen MK (eds) Taurine: biological actions and clinical perspectives. Liss, New York, pp 3–21Google Scholar
  6. Giri SN, Wang Q (1992) Taurine and niacin offer a novel therapeutic modality in prevention of chemically-induced pulmonary fibosis in hamsters. In: Lombardini JB, Schaffer SW, Azuma J (eds) Taurine: nutritional value and mechanisms of action. Plenum Press, New York, pp 329–340Google Scholar
  7. Gordon ER, Shaked AA Solano DF (1986) Taurine protects hamster bronchioles from acute NO,-induced alterations. Am J Pathol 66: 585–592Google Scholar
  8. Gordon RE, Heller RF, Heller RF (1992) Taurine protection of lungs in hamster models of oxidant injury: a morphologic time study of paraquat and bleomycin treatment. In: Lombardini JB, Schaffer SW, Azuma J (eds) Taurine: nutritional value and mechanisms of action. Plenum Press, New York, pp 319–328Google Scholar
  9. Hamaguchi T, Azuma J, Schaffer SW (1991) Interaction of taurine with methionine: inhibition of myocardial phospholipid methyltransferase. J Cardiovas Pharmacol 19: 224–230Google Scholar
  10. Harada H, Allo S, Viyuoh N, Azuma J, Takahashi K, Schaffer SW (1988) Regulation of calcium transport in drug-induced taurine-depleted hearts. Biochim Biophys Acta 944: 273–278Google Scholar
  11. Hussain MZ, Giri SN, Bhatnagar RS (1985) Poly (ADP-ribose) synthetase activity during bleomycin-induced lung fibrosis in hamsters. Exp Mol Pathol 43: 162–176Google Scholar
  12. Huxtable RJ (1989) Taurine in the central nervous system and the mammalian actions of taurine. Progress Neurobiol 32: 471–476Google Scholar
  13. Huxtable RJ (1992) Physiological actions of taurine. Physiol Rev 72: 101–105Google Scholar
  14. Kennedy TP, Gordon GB, Paley A, McShane A, Adkinson NF Jr, Peters SP, Friday K, Jackman W, Sciuto AM, Gurtner GH (1988) Amiodarone causes acute oxidant lung injury in ventilated and perfused rabbit lungs. J Cardiovas Pharmacol 12: 23–36Google Scholar
  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1955) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  16. Lombardini JB (1991) Taurine: retinal function. Brain Res Rev 16: 151–169Google Scholar
  17. Miles PR, Castranova V, Lee P (1978) Reactive forms of oxygen and chemiluminescence in phagocytizing rabbit alveolar macrophages. Am J Physiol 235: C103-C108Google Scholar
  18. Mozaffari MS, Tan BH, Lucia MA, Schaffer SW (1986) Effect of drug-induced taurine depletion on cardiac contractility and metabolism. Biochem Pharmacol 35: 985–989Google Scholar
  19. Schaffer SW, Azuma J (1992) Review: myocardial physiological effects of taurine and their significance. In: Lombardini JB, Schaffer SW, Azuma J (eds) Taurine: nutritional value and mechanisms of action. Plenum Press, New York, pp 105–120Google Scholar
  20. Schuller-Levis GB, Sturman JA (1992) Activation of alveolar leukocytes isolated from cats fed taurine-free diets. In: Lombardini JB, Schaffer SW, Azuma J (eds) Taurine: nutritional value and mechanisms of action. Plenum Press, New York, pp 83–90Google Scholar
  21. Spitz DR, Oberley LW (1989) An assay for superoxide dismutase activity in mammalian tissue homogenates. Analytical Biochem 179: 8–18Google Scholar
  22. Sturman JA (1993) Taurine in development. Physiol Rev 73: 119–123Google Scholar
  23. Wang Q, Giri SN, Hyde DM, Nakashima JM (1989) Effects of taurine on bleomycininduced lung fibrosis in hamsters. Proc Soc Exp Biol Med 190: 330–338Google Scholar
  24. Wang Q, Giri SN, Hyde DM, Li C (1991) Amelioration of bleomycin-induced pulmonary fiborsis in hamsters by combined treatment with taurine and niacin. Biochem Pharmacol 42: 1115–1122Google Scholar
  25. Wang Q, Hyde DM, Giri SN (1992) Abatement of bleomycin-induced increases in vascular permeability, inflammatory cell infiltration, and fibrotic lesions in hamster lungs by combined treatment with taurine and niacin. Lab Invest 67: 234–242Google Scholar
  26. Wright CE, Tallan HH, Lin YY (1986) Taurine: biological update. Annu Rev Biochem 55: 427–453Google Scholar

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • X. Zhang
    • 1
  • J. B. Lombardini
    • 1
    • 2
  1. 1.Department of PharmacologyTexas Tech University Health Sciences CenterLubbockUSA
  2. 2.Ophthalmology & Visual SciencesTexas Tech University Health Sciences CenterLubbockUSA

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