Advertisement

Amino Acids

, Volume 15, Issue 1–2, pp 109–116 | Cite as

Renal excretory responses of taurine-depleted rats to hypotonic and hypertonic saline infusion

  • M. S. Mozaffari
  • B. K. Warren
  • J. Azuma
  • S. W. Schaffer
Full Papers

Summary

Male Wistar-Kyoto rats were given either tap water (control) or 3%β-alanine (taurine-depleted) for three weeks. To prepare for the kidney function studies, the animals were then implanted with femoral vessels and bladder catheters. Two days after surgery, each rat was given an intravenous infusion of saline at the rate of 50μl/min and urine samples were collected at specific time intervals. An isotonic saline solution (0.9% NaCl) was infused for determination of baseline parameters and was followed by the infusion of a hypotonic saline solution (0.45% NaCl). Two days later, the infusion protocol was repeated in the same animals; however, a hypertonic saline solution (1.8% NaCl) was substituted for the hypotonic saline solution. Renal excretion of fluid and sodium increased in the control, but not taurine-depleted, rats during the hypotonic saline infusion. Interestingly, diuretic and natriuretic responses were similar between the groups during hypertonic saline infusion. The results suggest that taurine-depletion in rats affects renal excretory responses to a hypotonic, but not a hypertonic, saline solution.

Keywords

Amino acids Taurine Rat Natriuresis Hypotonic saline Hypertonic saline 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amiry-Moghaddam M, Nagelhus E, Ottersen OP (1994) Light and electronmicroscopic distribution of taurine, an organic osmolyte, in rat renal tubule cells. Kidney Int 45: 10–22Google Scholar
  2. Burg MB (1995) Molecular basis of osmotic regulation. Am J Physiol 268 (6 pt 2): F983–996Google Scholar
  3. Fugelli K, Kanli H, Terreros DA (1995) Taurine efflux is a cell volume regulatory process in proximal renal tubules from the teleost Carassius auratus. Acta Physiol Scand 155 2: 223–232Google Scholar
  4. Galietta LJ, Falzoni S, Di Virgilio F, Romeo G, Zegarra-Moran O (1997) Characterization of volume-sensitive taurine- and Cl - permeable channels. Am J Physiol 273 (1 pt 1): C57-C66Google Scholar
  5. Huang CC, Basavappa S, Ellory JC (1996) Volume-activated taurine permeability in cells of the human erythroleukemic cell line K562. J Cellular Physiol 167 2: 354–358Google Scholar
  6. Jones DP, Miller LA, Chesney RW (1990) Adaptive regulation of taurine transport in two continuous renal epithelial cell lines. Kidney Int 38: 219–226Google Scholar
  7. McManus ML, Churchwell KB, Strange K (1995) Regulation of cell volume in health and disease. N Engl J Med 333: 1260–1266Google Scholar
  8. Miyata S, Matsushima O, Hatton GI (1997) Taurine in rat posterior pituitary: localization in astrocytes and selective release by hypoosmotic stimulation. J Comp Neurology 381: 513–523Google Scholar
  9. Moran J, Maar TE, Pasantes-Morales H (1994) Impaired cell volume regulation in taurine deficient cultured astrocytes. Neurochem Res 19 4: 415–420Google Scholar
  10. Mozaffari MS, Azuma J, Patel C, Schaffer SW (1997) Renal excretory responses to saline load in the taurine-depleted and the taurine-supplemented rat. Biochem Pharmacol 54: 619–624Google Scholar
  11. Nakanishi T, Takamitsu Y, Sugita M (1994) Role of taurine in the kidney: osmoregulatory taurine accumulation in renal medulla. Ad Exp Med Biol 359: 139–148Google Scholar
  12. Pasantes-Morales H, Martin del Rio (1990) Taurine and mechanisms of cell volume regulation. In: Taurine: functional neurochemistry, physiology and cardiology. WileyLiss, Inc., New York, pp 317–328Google Scholar
  13. Shaffer JE (1979) The effect of beta alanine and other inhibitors of taurine transport on taurine disposition. Ph.D Thesis, Thomas Jefferson University, PhiladelphiaGoogle Scholar
  14. Trachtman H, Lu P, Sturman JA (1993) Immunohistochemical localization of taurine in rat renal tissue: studies in experimental disease states. J Histochem Cytochem 41: 1209–1216Google Scholar
  15. Trachtman H, Yancey PH, Gullans SR (1995) Cerebral cell volume regulation during hypernatremia in developing rats. Brain Res 693/1–2: 155–162Google Scholar
  16. Uchida S, Nakanishi T, Kwon HM, Preston AS, Handler JS (1991) Taurine behaves as an osmolyte in Marin-Darby canine kidney cells: protection by polarized, regulated transport of taurine. J Clin Invest 88: 656–662Google Scholar

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • M. S. Mozaffari
    • 1
  • B. K. Warren
    • 1
  • J. Azuma
    • 2
  • S. W. Schaffer
    • 3
  1. 1.Department of Oral BiologyMedical College of Georgia School of DentistryAugustaUSA
  2. 2.Department of Clinical Evaluation of Medicines and Therapeutics, Faculty of Pharmaceutical SciencesOsaka UniversityOsakaJapan
  3. 3.Department of Pharmacology, College of MedicineUniversity of South AlabamaMobileUSA

Personalised recommendations