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Amino Acids

, Volume 15, Issue 1–2, pp 77–88 | Cite as

Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells

  • T. E. Maar
  • T. M. Lund
  • G. Gegelashvili
  • R. Hartmann-Petersen
  • J. Moran
  • H. Pasantes-Morales
  • V. Berezin
  • E. Bock
  • Arne Schousboe
Full Papers

Summary

Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2–5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2–5 mM GES plus 2–5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.

Keywords

Amino acids Taurine Neurons NCAM Migration Transcription 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • T. E. Maar
    • 1
  • T. M. Lund
    • 1
  • G. Gegelashvili
    • 1
  • R. Hartmann-Petersen
    • 2
  • J. Moran
    • 3
  • H. Pasantes-Morales
    • 3
  • V. Berezin
    • 2
  • E. Bock
    • 2
  • Arne Schousboe
    • 1
  1. 1.PharmaBiotec Research Center, The Neurobiolog Unit, Department of PharmacologyThe Royal Danish School of PharmacyCopenhagenDenmark
  2. 2.Center for Medical Biotechnology, Institute of Molecular Pathology, Protein Laboratory, Panum InstituteUniversity of CopenhagenDenmark
  3. 3.Institute of Cellular Physiology, Department of NeurosciencesNational University of MexicoMexico CityMexico

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