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Amino Acids

, Volume 15, Issue 1–2, pp 43–52 | Cite as

Activation of adenosine A2 receptors enhances high K+-evoked taurine release from rat hippocampus: A microdialysis study

  • Junichi Hada
  • T. Kaku
  • K. Morimoto
  • Y. Hayashi
  • K. Nagai
Full Papers

Summary

The present study was designed to examine which type of adenosine receptors was involved in enhancement of high K+-evoked taurine release fromin vivo rat hippocampus using microdialysis. Perfusion with 0.5 or 5.0 mM adenosine enhanced high K+-evoked taurine release. Perfusion with 2μM R(−)-N6-2-phenylisopropyladenosine (PIA), a selective adenosine A1 receptor agonist, did not modulate taurine release. Perfusion with 1μM 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), a selective adenosine A1 receptor antagonist, increased taurine release. On the other hand, perfusion with 20μM 2-[4-(2-carboxyethyl)phenethylamino]-5′-N-ethyl-carboxamideadenosine (CGS21680), a selective adenosine A2A receptor agonist, enhanced taurine release, while perfusion with 1 mM 3,7-dimethyl-propagylxanthine (DMPX), an adenosine A2 receptor antagonist, did not affect taurine release. These results demonstrate that adenosine enhances high K+-evoked taurine release via activation of adenosine A2A receptors from both neurons and glial cells ofin vivo rat hippocampus.

Keywords

Amino acids Adenosine Taurine Hippocampus Microdialysis Spreading depression 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Junichi Hada
    • 1
  • T. Kaku
    • 1
  • K. Morimoto
    • 3
  • Y. Hayashi
    • 1
  • K. Nagai
    • 2
  1. 1.Department of PhysiologyHyogo College of MedicineHyogoNishinomiyaJapan
  2. 2.Department of PharmacologyHyogo College of MedicineHyogoJapan
  3. 3.Department of NeurosurgeryOsaka Medical Center and Research Institute for Maternal and Child HealthOsakaJapan

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