Skip to main content
Log in

Activation of adenosine A2 receptors enhances high K+-evoked taurine release from rat hippocampus: A microdialysis study

  • Full Papers
  • Published:
Amino Acids Aims and scope Submit manuscript

Summary

The present study was designed to examine which type of adenosine receptors was involved in enhancement of high K+-evoked taurine release fromin vivo rat hippocampus using microdialysis. Perfusion with 0.5 or 5.0 mM adenosine enhanced high K+-evoked taurine release. Perfusion with 2μM R(−)-N6-2-phenylisopropyladenosine (PIA), a selective adenosine A1 receptor agonist, did not modulate taurine release. Perfusion with 1μM 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), a selective adenosine A1 receptor antagonist, increased taurine release. On the other hand, perfusion with 20μM 2-[4-(2-carboxyethyl)phenethylamino]-5′-N-ethyl-carboxamideadenosine (CGS21680), a selective adenosine A2A receptor agonist, enhanced taurine release, while perfusion with 1 mM 3,7-dimethyl-propagylxanthine (DMPX), an adenosine A2 receptor antagonist, did not affect taurine release. These results demonstrate that adenosine enhances high K+-evoked taurine release via activation of adenosine A2A receptors from both neurons and glial cells ofin vivo rat hippocampus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bourke RS, Kimelberg HK, Daza MA (1978) Effects of inhibitors and adenosine on (HCO3 /CO2)-stimulated swelling and Cl uptake in brain slices and cultured astrocytes. Brain Res 154: 196–202

    Google Scholar 

  • Bourke RS, Waldman JB, Kimelberg HK, Barron KD, uSan Filippo BD, Popp AJ, Nelson LR (1981) Adenosine-stimulated astroglial swelling in cat cerebral cortexin vivo with total inhibition by a non-diuretic acylaryloxyacid derivative. J Neurosurg 55: 364–370

    Google Scholar 

  • Brundege JM, Dunwiddie TV (1997) Role of adenosine as a modulator of synaptic activity in the central nervous system. Adv Pharmacol 39: 353–391

    Google Scholar 

  • Cunha RA, Johansson B, Constantino MD, Sebastiao AM, Fredholm BB (1996) Evidence for high-affinity binding sites for the adenosine A2A receptor agonist [3H]CGS21680 in the rat hippocampus and cerebral cortex that are different from striatal A2A receptors. Naunyn-Schmiedeberg's Arch Pharmacol 353: 261–271

    Google Scholar 

  • Fredholm BB, Abbracchio MP, Burnstock G, Daly JW, Harden TK, Jacobson KA, Leff P, Williams M (1994) Nomenclature and classification of purinoceptors. Pharmacol Rev 46: 143–156

    Google Scholar 

  • Hada J, Kaku T, Morimoto K, Hayashi Y, Nagai K (1995) Effects of L-PIA, an adenosine A1 receptor agonist, on high K+-evoked amino acid release and spreading depression. 4th IBRO World Congress of Neuroscience [Abstract]: 193

  • Hada J, Kaku T, Morimoto K, Hayashi Y, Nagai K (1996) Adenosine transport inhibitors enhance high K+-evoked taurine release from rat hippocampus. Eur J Pharmacol 305: 101–107

    Google Scholar 

  • Huxtable RJ (1992) Physiological actions of taurine. Physiol Rev 72: 101–163

    Google Scholar 

  • Kaku T, Hada J, Hayashi Y (1994) Endogenous adenosine exerts inhibitory effects upon the development of spreading depression and glutamate release by microdialysis with K+ in rat hippocampus. Brain Res 658: 39–48

    Google Scholar 

  • Kaku T, Hada J, Morimoto K, Hayashi Y (1997) Role of adenosine upon high K+-evoked spreading depression and glutamate release fromin vivo rat hippocampus. In: Okada Y(ed) The role of adenosine in the nervous system. Elsevier, Amsterdam, pp 157–164

    Google Scholar 

  • Kamisaki Y, Maeda K, Ishimura M, Omura H, Itoh T (1993) Effects of taurine on depolarization-evoked release of amino acids from rat cortical synaptosomes. Brain Res 627: 181–185

    Google Scholar 

  • Koyama Y, Ishibashi T, Tanaka K, Baba A (1994) L-Glutamate-stimulated taurine release from rat cerebral cultured astrocytes. J Neurosci Res 38: 75–80

    Google Scholar 

  • Madelian V, Silliman S, Shain W (1988) Adenosine stimulates cAMP-mediated taurine release from LRM55 glial cells. J Neurosci Res 20: 176–181

    Google Scholar 

  • Menendez N, Solis JM, Herreras O, Herranz AS, Martin del Rio R (1990) Role of endogenous taurine on the glutamate analogue-induced neurotoxicity in the rat hippocampus in vivo. J Neurochem 55: 714–717

    Google Scholar 

  • Miyamoto AT, Miyamoto JK (1996) Effects of adenosine on taurine release in the central nervous system. Jpn J Physiol 46 [Suppl]: S179

    Google Scholar 

  • Saransaari P, Oja SS (1992) Release of GABA and taurine from brain slices. Prog Neurobiol 38: 455–482

    Google Scholar 

  • Schousboe A, Pasantes-Morales H (1989) Potassium-stimulated release of [3H]taurine from cultured GABAergic and glutamatergic neurons. J Neurochem 53: 1309–1315

    Google Scholar 

  • Somjen GG, Aitken PG, Czeh GL, Herreras O, Jing J, Young JN (1992) Mechanisms of spreading depression: a review of recent findings and a hypothesis. Can J Physiol Pharamacol 70: S248-S254

    Google Scholar 

  • Taber KH, Lin C-T, Liu J-W, Thalmann RH, Wu J-Y (1986) Taurine in hippocampus: localization and postsynaptic action. Brain Res 386: 113–121

    Google Scholar 

  • uVon Lubitz DKJE, Carter M, Beenhakker M, Lin RC-S, Jacobson KA (1995) Adenosine: a prototherapeutic concept in neurodegeneration. Ann NY Acad Sci 765: 163–178

    Google Scholar 

  • Zeise M (1985) Taurine on hippocampal slices: comparison to GABA and glycine, and antagonism by 4-aminopyridine. In: Oja SS, Ahtee L, Kontro P, Paasonen MK (eds) Taurine: biological actions and clinical perspectives. Alan R. Liss, New York, pp 281–287

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hada, J., Kaku, T., Morimoto, K. et al. Activation of adenosine A2 receptors enhances high K+-evoked taurine release from rat hippocampus: A microdialysis study. Amino Acids 15, 43–52 (1998). https://doi.org/10.1007/BF01345279

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01345279

Keywords

Navigation