Abstract
The effects of different modulators on the phosphofructokinase (PFK) activity from the posterior adductor muscle of the sea musselMytilus galloprovincialis Lmk. were studied in mussels collected from N.W. Spain in spring/summer, 1988. Adenosine monophosphate (AMP), fructose 2,6-bisphosphate (Fru 2,6-P2) and ammonium ions individually activated PFK. AMP and Fru 2,6-P2 reduced ATP-mediated inhibition and the value ofS 0.5 [concentration of fructose 6-phosphate (Fru 6-P) for half-maximum velocity], whereas ammonium ions increasedV max (the rate at the saturating concentrations of Fru 6-P). Fru 2,6-P2 and ammonium ions both enhanced the effect of AMP, but in different ways: Fru 2,6-P2 reduced apparentK m for AMP (concentration of AMP for half-maximum activation) without affectingK m , whereas ammonium ions only altered the latter. The sharp increases in the values ofS 0.5 for Fru 6-P and of apparentK m for AMP that are caused by a drop in pH were reduced by the presence of Fru 2,6-P2, but not by ammonium ions. The results shows that Fru 2,6-P2 may contribute to maintain the PFK activity during hypoxia periods, whereas ammonium ions (in the presence of high AMP concentrations) may activate the enzyme during the first few hours of post-hypoxia re-immersion.
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Communicated by O. Kinne, Oldendorf/Luhe
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Villamarín, J.A., Ramos-Martínez, J.I. Effect of fructose 2,6-bisphosphate and ammonium ions on AMP-mediated activation of phosphofructokinase from the posterior adductor muscle of the sea musselMytilus galloprovincialis . Mar. Biol. 106, 235–238 (1990). https://doi.org/10.1007/BF01314805
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DOI: https://doi.org/10.1007/BF01314805