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Discharge characteristics of nematocysts isolated from acontia ofCalliactis parasitica

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Abstract

Experiments were performed on specimens ofCalliactis parasitica collected near Plymouth in April 1987 and in the Mediterranean between September 1987 and April 1988. Undischarged nematocysts (basitrichous isorhizas) were separated from the acontia of by using 1M glycerol, 1M citrate, 0.5% Nonidet or 0.5% Triton X-100 as isolating agents, or by freezing the acontial tissue. The mechanisms of extrusion were not studied. The effectiveness of 50 mM thioglycolate in discharging nematocysts isolated by the above methods and suspended in Ca- and Mg-free artificial sea water (ASW) was investigated. Nematocysts extruded in glycerol were more responsive to thioglycolate than those extruded in citrate. Capsules isolated in non-ionic detergents, however, and those obtained by freezing were not discharged by thioglycolate. If capsules extruded in glycerol were suspended in ASW and this was replaced with distilled water, they did not respond to thioglycolate. The effect of distilled water was only partly reversible. It was observed that these nematocysts, unlike those ofPelagia noctiluca andAiptasia mutabilis previously investigated, did not release measurable amounts of free Ca2+ during discharge. It is suggested that if calcium is not involved in the mechanism of discharge of isolatedC. parasitica nematocysts then some other ionic species may stabilize the resting condition of these capsules.

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Authors and Affiliations

  1. Institute of General Physiology, University of Messina, via Sperone 31, I-98010, Messina, Italy

    A. Salleo & G. La Spada

  2. Department of Pure and Applied Zoology, University of Reading, Whiteknights, P.O. Box 228, RG6 2AJ, Reading, England

    E. A. Robson

Authors
  1. A. Salleo
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  2. G. La Spada
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  3. E. A. Robson
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Communicated by M. Sará, Genova

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Salleo, A., La Spada, G. & Robson, E.A. Discharge characteristics of nematocysts isolated from acontia ofCalliactis parasitica . Mar. Biol. 104, 459–464 (1990). https://doi.org/10.1007/BF01314350

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  • DOI: https://doi.org/10.1007/BF01314350

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