Abstract
Following uptake of technetium-95m and americium-241 by lobsters (Homarus gammarus) from food, the subcellular distribution and binding in the major organ of accumulation, the digestive gland, were analysed.95mTc was predominantly bound to soluble proteins; approximately 40% was bound to two proteins displaying the same elution characteristics as Cdthioneins as indicated by gel permeation and ion-exchange chromatography, indicating reduction and sequestration by metallothionein in vivo. The identity of a third anionic low molecular weight Tc-binding component was not established. No evidence was found for binding of Tc to haemocyanin, ferritin or transferrin. Particulate Tc [probably TcO(OH)2] was bound to plasma membranes and was also present in lysosomes, indicating reduction of pertechnetate and pinocytosis in the digestive gland. In contrast,241Am was mainly sequestered by the lysosomal/vacuolar system, indicative of uptake by pinocytosis. Approximately 2 to 3% of the Am was bound to ferritin and 20% was bound to unidentified ligands of 10 and 20 kdaltons mol. wt. There was no evidence for sequestration of Am by metallothionein. These studies prove that waste radionuclides discharged by the nuclear industry are metabolically active and enter the normal metal metabolism and detoxication pathways of a marine shellfish.
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Literature cited
Barker, P. L., Gibson, R. (1977). Observations on the feeding mechanism, structure of the gut and digestive physiology of the european lobsterHomarus gammarus (L.). J. exp. mar. Biol. Ecol. 24: 297–924
Beasley, T. M., Lorz, H. V. (1986). A review of the biological and geochemical behaviour of technetium in the marine environment. In: Desmet, G., Myttenaere (eds.) Technetium in the environment. Elsevier, London, p. 197–215
Becker, G. L., Chen, C., Greenwalt, J. W., Lehninger, J. W. (1974). Calcium phosphate granules in the hepatopancreas of the blue crabCallinectes sapidus. J. Cell Biol. 61: 316–326
Bottke, W., Crichton, R. R. (1984). Vitellogenic ferritin ofLymnea stagnalis L. (Mollusca, Gastropoda) differs in structure from soma cell type ferritin. Comp. Biochem. Physiol. 77B: 57–61
Brouwer, M., Whaling, P., Engel, D. W. (1986). Copper-metallothioneins in the American lobster,Homarus americanus: potential role as Cu(I) donors to apohemocyanin. Envir. Hlth Perspectives 65: 93–100
Depledge, M. H., Bjerregaard, P. (1989). Haemolymph protein composition and copper levels in decapod crustaceans. Helgoländer Meeresunters. 43: 207–223
Engel, D. W., Brouwer, M. (1986). Cadmium and copper metallothioneins in the American lobster,Homarus americanus. Envir. Hlth Perspectives 65: 87–92
Galey, J., Goudard, F., Piéri, J., Fowler, S. W., Carvalho, F. P. (1983). Tissue and subcellular distribution of252Cf and241Am in the seastarMarthasterias glacialis. Mar. Biol. 75: 253–259
Galey, J., Goudard, F., Piéri, J., Germain, P., George, S. G. (1986).241Am binding-components in the digestive gland cells of the marine prosobranchLittorina littorea. Comp. Biochem. Physiol. 85A: 333–340
George, S. G. (1982). Subcellular accumulation and detoxication of metals in aquatic animals. In: Vernberg, W. B., Calabrese, A., Thurberg, F., Vernberg, E. J. (eds.) Physiological mechanisms of marine pollutant toxicity. Academic Press, New York, p. 9–52
George, S. G. (1983). Heavy metal detoxication in the musselMytilus edulis. Composition of Cd-containing kidney granules (tertiary lysosomes) Comp. Biochem. Physiol. 76C: 59–57
George, S. G. (1989). Biochemical and cytological assessments of metal toxicity in marine animals. In: Furness, R. W., Rainbow, P. S. (eds.) Heavy metals in the marine environment. CRC Press, Boca Raton, p. 123–142
George, S. G., Pirie, B. J. S., Coombs, T. L. (1976). The kinetics of accumulation and excretion of ferric hydroxide inMytilus edulis (L.) and its distribution in the tissues. J. exp. mar. Biol. Ecol. 223: 71–84
George, S. G., Viarengo, A. (1985). An integration of current knowledge of the uptake, metabolism and intracellular control of heavy metals in mussels. In: Vernberg, F. J., Thurberg, F. P., Calabrese, A., Vernberg, W. B. (eds.) Marine pollution and physiology: recent advances. University of South Carolina Press, Columbia, p. 125–144
Ghiretti-Magaldi, A., Nuzzolo, C., Ghiretti, F. (1966). Chemical studies on hemocyanins. I. Amino acid composition. Biochemistry (Am. chem. Soc.) Easton, Pa. 5: 1949–1951
Goudard, F., Galey, J., Pieri, J., Fowler, S. W., Heussner, S., La Rosa, J. (1985). Intracellular localization and binding of technetium-95m in the seastarMarthasterias glacialis. Mar. Biol. 85: 43–50
Gründer, R., Seidel, A., Winter, R. (1981). The initial early distribution of monomeric239Pu and241Am in rat liver as determined by Triton WR1339 injection. Radiat. Res. 85: 367–379
Guary, J. C. (1980). Recherches sur les transferts et la fixation du plutonium, de l'américium et du neptunium dans le milieu marin. Thèse de Docteur d'Etat-Sciences, Université Aix-Marseille II
Guary, J. G., Negrel, R. (1980). Plutonium and iron association with metal-binding proteins in the crabCancer pagurus L. J. exp. mar. Biol. Ecol. 42: 87–98
Guary, J. C., Negrel, R. (1981). Calcium phosphate granules: a trap for transuranics and iron in crab hepatopancreas. Comp. Biochem. Physiol. 68A: 429–427
Johanssen, b., Syhre, R., Spies, H., Munze, R. (1977). Chemical and biological characterization of different Tc complexes of cysteine and cysteine derivatives. J. nucl. Med. 19: 816–824
Kagi, J. H. R., Kojima, Y. (1987). Chemistry and biochemistry of metallothionein. Experientia (Suppl.) 52: 25–61
Keatch, H. W., Grant, P. T., George, S. G., Coombs, T. (1982). Iron-binding components in the digestive gland of the queen scallop,Chlamys opercularis (L.). Biochem. Soc. Trans. 10: 461–462
Lerch, K. R., Ammer, D., Olafson, R. W. (1982). Crab metallothionein. Primary structure of metallothioneins 1 and 2. J. biol. Chem. 257: 2420–2426
Martin, J. L. M., Van Wormhoudt, A., Ceccaldi, H. J. (1977). Zinc hemocyanin binding in the hemolymph ofCarcinus maenas Crustacea, Decapoda. Comp. Biochem. Physiol. 58A: 193–195
Morelock, M. M., Tolman, G. L. (1987). Metallothionein: a bifunctional chelator for the radiolabelling of biologically active molecules. Experientia (Suppl.) 52: 247–253
Overnell, J. (1984). The partition of copper and cadmium between different charge-forms of metallothionein in the digestive tubules of the crab,Cancer pagurus. Comp. Biochem. Physiol. 77C: 237–243
Pentreath, R. J. (1981). The biological availability to marine organisms of transuranium and other long-lived nuclides. In: Proceedings of Symposium on Impacts of Radionuclide Releases into the Marine Environment. International Atomic Energy Agency, Vienna, p. 241–272
Ray, S., White, M. (1981). Metallothionein-like protein in the lobstersHomarus americanus. Chemosphere (U.K.) 11/12: 1205–1219
Schneider, Y. P., Tulkens, P., De Duve, C., Trouet, A. (1979). Fate of plasma membrane during endocytosis. II. Evidence of recycling (shuttle) of plasma membrane constituents. J. Cell Biol. 82: 466–474
Senkbeil, E. G., Wriston, Jr., J. C. (1981). Hemocyanin synthesis in the American lobster,Homarus americanus. Comp. Biochem. Physiol. 68B: 169–171
Simkiss, K. (1977). Biomineralization and detoxification. Calcif. Tissue Res. 24: 199–200
Steinman, R. M., Mellmen, I. S., Muller, W. A., Cohn, Z. A. (1983). Endocytosis and the recycling of plasma membrane. J. Cell Biol. 96: 1–27
Stover, B. J., Breunger, F. W., Stevens, W. (1970). Association of americium with ferritin in the canine liver. Radiat. Res. 43: 173–186
Sutterlin, U., Thies, W. G., Haffner, H., Seidel, A. (1984). Comparative studies on the lysosomal association of monomeric239Pu and241Am in rat and chinese hamster liver: analysis with sucrose, metrizamide and percoll density gradients of subcellular binding as dependent upon time. Radiat. Res. 98: 293–306
Tan, A. T., Woodworth, R. C. (1969). Ultraviolet difference spectra studies of conalbumin complexes with transition metal ions. Biochemistry (Am. chem. Soc.) Easton, Pa. 8: 9711–9716
Taylor, D. M. (1972). Interactions between transuranium elements and the components of cells and tissues. Hlth Phys. 22: 575–581
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Communicated by J. Mauchline, Oban
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Goudard, F., Durand, J.P., Galey, J. et al. Subcellular localisation and identification of95mTc- and241Am-binding ligands in the hepatopancreas of the lobsterHomarus gammarus . Mar. Biol. 108, 411–417 (1991). https://doi.org/10.1007/BF01313650
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DOI: https://doi.org/10.1007/BF01313650