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Cytochrome P-450 monooxygenase system and benzo(a)pyrene metabolism in echinoderms

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Abstract

A comparative study on mixed-function oxygenase (MFO) systems was carried out on four echinoderm species: the asteroidsAsterias rubens andMarthasterias glacialis, a holothurianHolothuria forskali and an echinoidEchinus esculentus. Cytochromes P-450 andb 5 and the MFO system-associated NADH-ferricyanide reductases NADH-cytochromec reductase, NADPH-cytochromec reductase and benzo(a)pyrene (BP) hydroxylase activities were present in microsomal fractions of pyloric caeca ofA rubens andM. glacialis and in the haemal plexus ofH. forskali. In contrast, cytochrome P-450 and BP hydroxylase activity were not detectable in the gonads ofE. esculentus. The tissue and subcellular distribution of the MFO system was studied inA. rubens. MFO system components were found in the stomachs and gonads, although detection of cytochrome P-450 in the latter tissue was difficult. Sex-related differences were not significant. The contents of the MFO system components and BP hydroxylase activities were highest in the microsomal (100 000 ×g) fractions, but MFO system components were also found in the mitochondrial (12 000 ×g) and cytosolic fractions. The BP hydroxylase activity in pyloric caeca microsomes ofA. rubens was NADPH-dependent and was inhibited by several agents known to be inhibitors of vertebrate cytochromes P-450. In the former respect, the characteristics of the MFO system were more like those of vertebrates and crustaceans than that of molluscs.

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Authors and Affiliations

  1. Research Group for Aquatic Toxicology, University of Utrecht, P.O. Box 80058, N-3508, Utrecht, TB, The Netherlands

    P. J. den Besten, H. J. Herwig & E. G. van Donselaar

  2. Plymouth Marine Laboratory, Citadel Hill, PL1 2PB, Plymouth, UK

    D. R. Livingstone

Authors
  1. P. J. den Besten
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  2. H. J. Herwig
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  3. E. G. van Donselaar
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  4. D. R. Livingstone
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Communicated by O. Kinne, Oldendorf/Luhe

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den Besten, P.J., Herwig, H.J., van Donselaar, E.G. et al. Cytochrome P-450 monooxygenase system and benzo(a)pyrene metabolism in echinoderms. Mar. Biol. 107, 171–177 (1990). https://doi.org/10.1007/BF01313254

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