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Evaluation of three hepatitis C virus-related antibodies C100, KCL-163, JCC

Tests for screening blood donors

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Abstract

To evaluate the most effective method for detecting hepatitis C virus (HCV) carriers in a large population of blood donors, HCV-related antibodies were measured in 919 donor serum samples using three different enzyme-linked immunosorbent assays. The antibodies were C100 and KCL-163, nonstructural proteins of HCV, as well as JCC, a translation product of the presumptive HCV core gene. Fourteen (1.5%), 12 (1.3%), and 13 (1.4%) specimens were positive for anti-C100, anti-KCL-163, and anti-JCC, respectively. HCV RNA was detected by the polymerase chain reaction in seven (25.0%) of the 28 specimens that were anti-HCV-positive by at least one of the three assays. Four of the seven specimens were detected by anti-C100 screening, while the remaining three were not. All seven specimens were positive for KCL-163 and/or JCC antibodies. These findings suggest that screening for both KCL-163 and JCC antibodies may be of particular use in accurately identifying HCV-positive blood.

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References

  1. Prince AM, Brotman B, Grady GF, Kuhns WJ, Hazzi C, Levine RW, Millian SJ: Long-incubation posttransfusion hepatitis without serological evidence of exposure to hepatitis-B virus. Lancet 2:241–246, 1974

    Google Scholar 

  2. Choo Q-L, Kuo G, Weiner AJ, Overby LR, Bradley DW, Houghton M: Isolation of a cDNA clone derived from a blood-borne non-A, non-B hepatitis genome. Science 244:359–362, 1989

    Google Scholar 

  3. Kuo G, Choo Q-L, Alter HJ, Gitnick GL, Redeker AG, Purcell RH, Miyamura T, Dienstag JL, Alter MJ, Stevens CE, Tegtmeier GE, Bonino F, Colombo M, Lee W-S, Kuo C, Berger K, Shuster JR, Overby LR, Bradley DW, Houghton M: An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364, 1989

    Google Scholar 

  4. Kiyosawa K, Akahane Y, Nagata A, Furuta S: Hepatocellular carcinoma after non-A, non-B posttransfusion hepatitis. Am J Gastroenterol 79:777–781, 1984

    Google Scholar 

  5. Nishioka K, Watanabe J, Furuta S, Tanaka E, Iino S, Suzuki H, Tsuji T, Yano M, Kuo G, Choo Q-L, Houghton M, Oda T: A high prevalence of antibody to the hepatitis C virus in patients with hepatocellular carcinoma in Japan. Cancer 67:429–433, 1991

    Google Scholar 

  6. Nishioka K: Hepatitis C virus infection in Japan. Gastroenterol Jpn 26(suppl 3):152–155, 1991

    Google Scholar 

  7. Katayama T, Kikuchi S, Tanaka Y, Saito I, Miyamura T, Choo Q-L, Houghton M, Kuo G: Blood screening for non-A, non-B hepatitis by hepatitis C virus antibody assay. Transfusion 30:374–376, 1990

    Google Scholar 

  8. Inaba S, Fukuda M, Okochi K, Irita Y, Tokunaga K, Kiyokawa H, Maeda Y: HCV transmission after receiving anti-C100-negative blood units. Lancet 337:1354, 1991

    Google Scholar 

  9. Van der Poel CL, Reesink HW, Cuypers HTM, Lelie PN, Schaasberg W: Is anti-HCV blood donor screening useful? Lancet 336:1193, 1990

    Google Scholar 

  10. Miyamura T, Saito I, Katayama T, Kikuchi S, Tateda A, Houghton M, Choo Q-L, Kuo G: Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: Application to diagnosis and blood screening for posttransfusion hepatitis. Proc Natl Acad Sci USA 87:983–987, 1990

    Google Scholar 

  11. Weiner AJ, Kuo G, Bradley DW, Bonino F, Saracco G, Lee C, Rosenblatt J, Choo Q-L, Houghton M: Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet 335:1–3, 1990

    Google Scholar 

  12. Garson JA, Tedder RS, Briggs M, Tuke P, Glazebrook JA, Trute A, Parker D, Barbara JAJ, Contreras M, Aloysius S: Detection of hepatitis C viral sequences in blood donations by “nested polymerase chain reaction” and prediction of infectivity. Lancet 335:1419–1422, 1990

    Google Scholar 

  13. Kawano S, Fujiyama S, Sato S, Yoshida K, Shibata J, Murata H, Kawahara T, Mizuno K, Nonaka S, Sato T: Detection of antibodies to hepatitis C virus (anti-HCV) in patients with various liver diseases by an ELISA (KCL-163) test consisting of synthetic peptides corresponding to an HCV genome. Gastroenterol Jpn 26(suppl 3):218–220, 1991

    Google Scholar 

  14. Sato S, Fujiyama S, Kawano S, Sato T, Mizuno K, Nonaka S: Evaluation of the KCL-163 ELISA test for detecting antibodies against hepatitis C virus in patients with non-A, non-B chronic hepatitis. Gastroenterol Jpn 26(suppl 3):216–217, 1991

    Google Scholar 

  15. Kawano S, Fujiyama S, Sato S, Tanaka M, Goto M, Taura Y, Sato T, Kawahara T, Mizuno K, Nonaka S. Clinical evaluation of three anti-HCV ELISAs in patients with various liver diseases. Dig Dis Sci 37, 1992 (in press)

  16. Hiraga M, Hayashi N, Komatsu K, Hayashi K, Suzuki T, Kaminaka K, Sugimoto H, Higashi K, Kawahara T, Fujiyama S, Shikata T: A recombinant core protein corresponding to hepatitis C virus mutant expressed in Escherichia coli and the establishment of an ELISA system. Acta Hepatol Jap 32:315, 1991 (in Japanese)

    Google Scholar 

  17. Chiba J, Ohba H, Matsuura Y, Watanabe Y, Katayama T, Kikuchi S, Saito I, Miyamura T: Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus. Proc Natl Acad Sci USA 88:4641–4645, 1991

    Google Scholar 

  18. Mimms L, Vallari D, Ducharme L, Holland P, Kuramoto IK, Zeldis J: Specificity of anti-HCV ELISA assessed by reactivity to three immunodominant HCV regions. Lancet 336:1590–1591, 1990

    Google Scholar 

  19. Okamoto H, Okada S, Sugiyama Y, Tanaka T, Sugai Y, Akahane Y, Machida A, Mishiro S, Yoshizawa H, Miyakawa Y, Mayumi M: Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5′-noncoding region. Jpn J Exp Med 60:215–222, 1990

    Google Scholar 

  20. Van der Poel CL, Reesink HW, Schaasberg W, Leentvaar-Kuypers A, Bakker E, Exel-Oehlers PJ, Lelie PN: Infectivity of blood seropositive for hepatitis C virus antibodies. Lancet 335:558–560, 1990

    Google Scholar 

  21. Esteban JI, González A, Hernández JM, Viladomiu L, Sánchez C, López-Talavera JG, Lucea D, Martin-Vega C, Vidal X, Esteban R, Guardia J: Evaluation of antibodies to hepatitis C virus in a study of transfusion-associated hepatitis. N Engl J Med 323:1107–1112, 1990

    Google Scholar 

  22. Yoshizawa H: Blood transfusion and non-A, non-B hepatitis. Acta Hepatol Jap 32:221–223, 1991 (in Japanese)

    Google Scholar 

  23. Zanetti AR, Tanzi E, Zehender G, Magni E, Incarbone C, Zonaro A, Primi D, Cariani E: Hepatitis C virus RNA in symptomless donors implicated in posttransfussion non-A, non-B hepatitis. Lancet 336:448, 1990

    Google Scholar 

  24. Maéno M, Kaminaka K, Sugimoto H, Esumi M, Hayashi N, Komatsu K, Abe K, Sekiguchi S, Yano M, Mizuno K, Shikata T: A cDNA clone closely associated with non-A, non-B hepatitis. Nucleic Acids Res 18:2685–2689, 1990

    Google Scholar 

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Authors and Affiliations

  1. Third Department of Internal Medicine, Kumamoto University Medical School, 1-1, Honjo 1-chome, Kumamoto 860, Japan

    Shigetoshi Fujiyama MD, Shin-Ichi Kawano MD, Shinjiro Sato MD, Tatsuo Sato MD, Tetsuo Kawahara MS, Kyosuke Mizuno DVM, Yukihiko Kusumoto MD, Mariko Esumi PhD & Toshio Shikata MD

  2. The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan

    Shigetoshi Fujiyama MD, Shin-Ichi Kawano MD, Shinjiro Sato MD, Tatsuo Sato MD, Tetsuo Kawahara MS, Kyosuke Mizuno DVM, Yukihiko Kusumoto MD, Mariko Esumi PhD & Toshio Shikata MD

  3. Kumamoto Red Cross Blood Center, Kumamoto, Japan

    Shigetoshi Fujiyama MD, Shin-Ichi Kawano MD, Shinjiro Sato MD, Tatsuo Sato MD, Tetsuo Kawahara MS, Kyosuke Mizuno DVM, Yukihiko Kusumoto MD, Mariko Esumi PhD & Toshio Shikata MD

  4. First Department of Pathology, Nihon University, Tokyo, Japan

    Shigetoshi Fujiyama MD, Shin-Ichi Kawano MD, Shinjiro Sato MD, Tatsuo Sato MD, Tetsuo Kawahara MS, Kyosuke Mizuno DVM, Yukihiko Kusumoto MD, Mariko Esumi PhD & Toshio Shikata MD

Authors
  1. Shigetoshi Fujiyama MD
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  2. Shin-Ichi Kawano MD
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  3. Shinjiro Sato MD
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  4. Tatsuo Sato MD
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  5. Tetsuo Kawahara MS
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  6. Kyosuke Mizuno DVM
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  7. Yukihiko Kusumoto MD
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  8. Mariko Esumi PhD
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  9. Toshio Shikata MD
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This work was supported in part by a grant from the Sagawa Science Foundation.

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Fujiyama, S., Kawano, SI., Sato, S. et al. Evaluation of three hepatitis C virus-related antibodies C100, KCL-163, JCC. Digest Dis Sci 37, 1489–1494 (1992). https://doi.org/10.1007/BF01296491

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  • DOI: https://doi.org/10.1007/BF01296491

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