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Sunflower cytosolic-glutamine-synthetase genes showing similar organ-specificity patterns exhibit very different expression intensities by reverse-transcription polymerase chain reaction and Northern analysis

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Summary

Four genes coding for the cytosolic isoforms of glutamine synthetase (GS; EC 6.3.2.1) were recently identified in sunflower. The levels of transcripts from those genes have been analyzed in selected organs where ammonium is being produced by different processes that might differentially regulate the genes' expression. Genes exhibiting consistently high (ggs1.1 and−1.4), low (ggs1.2), and very low (ggs1.3) expression have been found. However, the four cytosolic-GS genes were expressed according to very similar patterns of organ specificity suggesting a low degree of specialization within this gene family in sunflower.

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Authors and Affiliations

  1. División de Fisiologéa Vegetal, Facultad de Ciencias, Universidad de Córdoba, E-14071, Córdoba, Spain

    M. Montenegro, J. M. Maldonado & R. Pérez-Vicente

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  1. M. Montenegro
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  2. J. M. Maldonado
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  3. R. Pérez-Vicente
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Correspondence to R. Pérez-Vicente.

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Montenegro, M., Maldonado, J.M. & Pérez-Vicente, R. Sunflower cytosolic-glutamine-synthetase genes showing similar organ-specificity patterns exhibit very different expression intensities by reverse-transcription polymerase chain reaction and Northern analysis. Protoplasma 207, 154–157 (1999). https://doi.org/10.1007/BF01282995

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  • DOI: https://doi.org/10.1007/BF01282995

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