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Embryonic platelet activating factor production in the rabbit increases during the preimplantation phase

  • Animal Investigations
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Objective

This study measured platelet activating factor (PAF) production by rabbit embryos in vitro and ascertained if there is increased PAF production associated with advancing embryonic development.

Study Design

Two-cell rabbit embryos were recovered from superovulated New Zealand White does and cultured in vitro for 96 hr. Every 24 hr embryos were scored for developmental stage and PAF activity from the corresponding culture medium was measured by platelet aggregation and organic phosphate analyses.

Results

PAF was detected in culture medium at all stages from two cells to blastocysts and rose significantly (P <0.001) at each 24-hr interval, reaching maximal levels at the expanded blastocyst stage.

Conclusion

Maximal PAF production by expanded blastocysts may be an embryonic paracrine signal that facilitates implantation.

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Authors and Affiliations

  1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, The University of Tennessee, Memphis, 956 Court Avenue, Room D-328, 38163, Memphis, Tennessee, USA

    Brijinder S. Minhas, Yan-Ping Zhu, Hak-Nam Kim, Thomas H. Burwinkel, Barry A. Ripps & John E. Buster

Authors
  1. Brijinder S. Minhas
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  2. Yan-Ping Zhu
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  3. Hak-Nam Kim
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  4. Thomas H. Burwinkel
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  5. Barry A. Ripps
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  6. John E. Buster
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Minhas, B.S., Zhu, YP., Kim, HN. et al. Embryonic platelet activating factor production in the rabbit increases during the preimplantation phase. J Assist Reprod Genet 10, 366–370 (1993). https://doi.org/10.1007/BF01213432

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  • DOI: https://doi.org/10.1007/BF01213432

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