Purpose
In order to determine an optimal marker to discriminate embryo injury following single-blastomere embryo biopsy, mouse embryos were examined for rates of blastocyst formation, hatching, implantation, and fetal development following single-blastomere biopsy.
Results
Early studies of single-blastomere biopsy (1–8 series) resulted in similar rates of blastocyst formation (P >0.05) but a lower rate of hatching of biopsied (n =140) versus control (nonbiopsied) (n =145) embryos (78.6 vs 95.2%; p <0.01). Subsequent experience (9–13 series) eliminated this difference between biopsied (n =145) and control embryos (n =133) (95.9 vs 94.0%; P >0.05). Embryo transfer of hatching blastocysts of biopsied (n =100) and nonbiopsied control (n =100) groups resulted in equivalent rates of implantation (96.0 vs 92.0%; p >0.05) and an equivalent rate of fetal development (70.0 vs 68.0%; p >0.05).
Conclusions
The hatching rate appeared to be a simple, sensitive, and reliable method to evaluate the singleblastomere biopsy technique.
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Cui, KH., Verma, P.J. & Matthews, C.D. Hatching rate: An optimal discriminator for the assessment of single-blastomere biopsy. J Assist Reprod Genet 10, 157–162 (1993). https://doi.org/10.1007/BF01207740
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DOI: https://doi.org/10.1007/BF01207740