Purpose
High-quality motile human spermatozoa were obtained following treatment of semen by simultaneous swim-up into medium and swim-down into an isotonic 40% Percoll solution.
Results
This procedure was significantly better than the swim-up method and comparable to discontinuous Percoll gradient centrifugation. Recovery rates of motile sperm were 35% for swim-up, 65% for Percoll gradient centrifugation, and 73% for swim-up/swim-down.
Conclusion
The swim-down sperm was inferior to the swim-up sperm in its upward migration capacity but superior in morphology. Spermatozoa obtained by the swim-up/swim-down procedure demonstrated fertilizing ability in IVF, and clinical pregnancies were established. The simultaneous swim-up/swim-down procedure offers an alternative efficient method of simple separation of high-quality motile sperm for various assisted reproduction techniques.
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Almagor, M., Benbenishti, D., Rosenak, D. et al. Simultaneous swim-up/swim-down of sperm in assisted reproduction procedures. J Assist Reprod Genet 10, 261–265 (1993). https://doi.org/10.1007/BF01204939
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DOI: https://doi.org/10.1007/BF01204939