Abstract
Tripronuclear zygotes obtained from a clinical IVF program were studied cytogenetically. Successful analysis was possible of 42 specimens at the zygote stage and 21 embryos after the first or second cleavage division. In the majority of zygotes (88%) the expected triploidy was confirmed, whereas only 14% of embryos had solely triploid cells. Therefore it is concluded that after tripolar cleavage division, many different types of mosaicism may originate from irregular chromosome distributions. Since the findings in individual blastomeres in embryos resulting from multipronuclear zygotes do not reflect the genetic content of the whole embryo, these embryos are less suitable in a model system for preimplantion diagnosis. The distribution of the sex chromosomal types (XXX, XXY, and XYY) confirmed theoretical expectations. Since in abortion material or in liveborn triploidy cases, the XYY karyotype is hardly ever observed, this indicates that most likely the 69,XYY karyotype has a very high embryonic mortality.
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Chatterjee MS, Tejani NA, Verma UL, Weiss RR: Prenatal diagnosis of triploidy. Int J Gynaecol Obstet 1983;21:155–157
Monrozies X, Monrozies M, Parinaud J, Berrebi A: La triploidie. J Gynecol Obstet Biol Reprod Paris 1986;15:893–900
Jacobs PA, Angell RR, Buchanan IM, Hassold TJ, Matsuyama AM, Manuel B: The origin of human triploids. Ann Hum Genet (Lond) 1978;42:49–57
Kola I, Trounson A: Dispermic human fertilization: Violation of expected cell behavior.In The Cell Biology of Fertilization, H Schatten, G Schatten (eds). San Diego, Academic Press, 1989, pp 277–293
Edwards RG: The case for studying human embryos and their constituent tissues in vitro.In Human Conception in Vitro. Proceedings of the First Bourn Hall Meeting, RG Edwards, JM Purdy (eds). London, Academic Press, 1982, pp 370–388
Angell RR, Templeton AA, Messinis IE: Consequences of polyspermy in man. Cytogenet Cell Genet 1986;42:1–7
Mahadevan MM, Trounson AO: The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984;42:400–405
Rudak E, Dor J, Mashiach S, Nebel L, Goldman B: Chromosome analysis of multipronuclear human oocytes fertilized in vitro. Fertil Steril 1984;41:538–545
Michelmann HW, Bonhoff A, Mettler L: Chromosome analysis in polyploid human embryos. Hum Reprod 1986;1:243–246
Wentz CA, Repp JE, Maxson WS, Pittaway DE, Torbit CA: The problem of polyspermy in in vitro fertilization. Fertil Steril 1983;40:748–754
Macas E, Suchanek E, Grizelji V, Puharic I, Simunic V: Chromosomal preparations of human triploid zygotes and embryos fertilized in vitro. Eur J Obstet Gynecol Reprod Biol 1988;29:299–304
Plachot M, Veiga A, Montagut J, de Grouchy J, Calderon G, Lepretre S, Junca A-M, Santalo J, Carles E, Mandelbaum J, Barri P, Degoy J, Cohen J, Egozcue J, Sabatier JC, Salat-Baroux J: Are clinical and biological IVF parameters correlated with chromosomal disorders in early life: A multicentric study. Hum Reprod 1988;3:627–635
Al-Hasani S, Ven van de H, Diedrich K, Hamerich U, Lehmann F, Krebs D: Polyploidien bei der In-vitro-fertilisation menschlicher Eizellen: Häufigkeit und mögliche Ursachen. Geburtsh Frauenheilk 1984;44:395–399
Evers JLH, Falck Larsen J, Gnany GG, Sieck UV: Complications and problems in transvaginal sector scan-guided follicle aspiration. Fertil Steril 1988;49:278–282
Quinn P, Warnes GM, Kerin JF, Kirby C: Culture factors in relation to the success of human in vitro fertilization and embryo transfer. Fertil Steril 1984;41:202–209
Dumoulin JCM, Evers JLH, Offermans JPM, Bras M, Pieters MHEC, Geraedts JPM: Human in vitro fertilization using spermatozoa capacitated in hyperosmotic media. Gynecol Obstet Invest 1990;30:165–168
Tarkowski AK: An air-drying method for chromosome preparation from mouse eggs. Cytogenetics 1966;5:394–400
Pieters MHEC, Geraedts JPM, Dumoulin JCM, Evers JLH, Bras M, Kornips FHAC, Menheere PPCA: Cytogenetic analysis of in vitro fertilization (IVF) failures. Hum Genet 1989;81:367–370
Seabright M: Rapid banding technique for human chromosomes. Lancet 1971;2:971–972
Schweizer D, Ambros P, Andrle M: Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding olipeptide antibiotic, dystamicin A. Exp Cell Res 1978;111:327–332
Plachot M, Mandelbaum J, Junca A, de Grouchy J, Salat-Baroux J, Cohen J: Cytogenetic analysis and developmental capacity of normal and abnormal embryos after IVF. Hum Reprod 1989;4 (Suppl):99–103
Kola I, Trounson A, Dawson G, Rogers P: Tripronuclear human oocytes: Altered cleavage patterns and subsequent karyotypic analysis of embryos. Biol Reprod 1987;37:395–401
Austin CR, Walton A: Fertilization.In Marshall's Physiology of Reproduction, AS Parkes (ed). London, Longmans, 1960, pp 310–416
Wilson EB: The Cell in Development and Heredity. New York, Macmillan 1928, pp 916–979
Reik W: Genomic imprinting and genetic disorders in man. Hum Genet Dis 1989;5:331–336
Van Blerkom J, Bell H, Henry G: The occurrence, recognition and developmental fate of pseudo-multipronuclear eggs after in-vitro fertilization of human oocytes. Hum Reprod 1987;2:217–225
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Pieters, M.H.E.C., Dumoulin, J.C.M., Ignoul-Vanvuchelen, R.C.M. et al. Triploidy after in vitro fertilization: Cytogenetic analysis of human zygotes and embryos. J Assist Reprod Genet 9, 68–76 (1992). https://doi.org/10.1007/BF01204118
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DOI: https://doi.org/10.1007/BF01204118