Abstract
The zona-free hamster egg test was carried out using spermatozoa from 15 men which consistently failed to fertilize their wives' oocytes in vitro. Spermatozoa from nine of these men fertilized hamster eggs in vitro, indicating that positive results in this assay are an unreliable guide to human in vitro fertilization. Donor spermatozoa were needed to fertilize the wife's oocytes in three of these cases. Nevertheless, the proportion of hamster egg penetration was significantly lower compared with spermatozoa from 15 men who could fertilize their wives' oocytes in vitro. The hamster assay also failed to indicate the establishment of pregnancy.
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Rogers BJ, van Campen H, Ueno M, Lambert H, Bronson R, Hale R: Analysis of human spermatozoal fertilizing ability using zona-free ova. Fertil Steril 1979;32:664–670
Cohen J, Weber RFA, van der Vijver JCM, Zeilmaker GH: In vitro fertilizing capacity of human spermatozoa with the use of zona-free hamster ova: Interassay variation and prognostic value. Fertil Steril 1982;37:565–572.
Cohen J, Mooyaart M, Vreeburg JTM, Zeilmaker GH: Fertilization of hamster ova by human spermatozoa in relation to other semen parameters. Int J Androl 1982;5:210–224
Aitken RJ, Best FSM, Richardson DW, Djahanbakhch O, Mortimer D, Templeton AA, Lees MM: An analysis of sperm function in cases of explained infertility: Conventional criteria, movement characteristics, and fertilizing capacity. Fertil Steril 1982;38:212–221
Edwards RG, Fishel SB, Purdy JM: In vitro fertilization of human eggs: Analysis of follicular growth, ovulation and fertilization.In Fertilization of the Human Egg in Vitro, HM Beier, HR Lindner, (eds), Berlin, Springer-Verlag, 1983, pp 169–188
Edwards RG, Fishel SB, Cohen J, Fehilly CB, Purdy JM, Slater J, Steptoe PC, Webster JM: Factors influencing the success of in-vitro fertilization for alleviating human infertility. J Vitro Fert Embryo Transfer 1984;1:3–23
Cohen J: Survival and fertilizing capacity of human spermatozoa in vitro at different temperatures (unpublished)
Cohen J, Mooyaart M, Vreeburg JTM, Yanagimachi R, Zeilmaker GH: Fertilizing ability and motility of spermatozoa from fertile and infertile men after exposure to heterologous seminal plasma.In Instrumental insemination, Clinics in Andrology, Vol 8, ESE Hafez, K Semm (eds). The Hague, Martinus Nyhoff, 1982, pp 53–62
Walters DE: Sampling the randomization distribution. Statistician 1981;30:289–295
Wolf DP, Sokoloski JE, Quigley MM: Correlation of human in vitro fertilization with the hamster egg bioassay. Fertil Steril 1983;40:53–59
Margalioth EJ, Navot D, Laufer N, Mor Yosef S, Rabinowitz R, Yarkoni S, Schenker JG: Zona free hamster ovum penetration assay as a screening procedure for in vitro fertilization. Fertil Steril 1983;40:386–388
Rogers BJ, Perreault SD, Bentwood BJ, McCarville C, Hale RW, Soderdahl DW: Variability in the human-hamster in vitro assay for fertility evaluation. Fertil Steril 1983;39: 204–211
Overstreet JW, Yanagimachi R, Katz DF, Hayashi K, Hanson FW: Penetration of human spermatozoa in the human zona pellucida and the zona-free hamster egg: A study of fertile donors and infertile patients. Fertil Steril 1980;33:534–542.
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Foreman, R., Cohen, J., Fehilly, C.B. et al. The application of the zona-free hamster egg test for the prognosis of human in vitro fertilization. J Assist Reprod Genet 1, 166–171 (1984). https://doi.org/10.1007/BF01139209
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DOI: https://doi.org/10.1007/BF01139209