Abstract
Eighteen two- to four-cell embryos, cultured in vitro for 32–65 hr after insemination, were examined by transmission electron microscopy to assess their normality and developmental potential. These stages are now being widely used for embryo transfer in in vitro fertilization clinics. They were obtained by inseminating preovulatory oocytes aspirated at laparoscopy, with or without ovarian stimulation, by methods which have yielded normal pregnancies. The organization of seven embryos was apparently normal and their blastomeres had cellular organelles usually present in fertilized ova. Details of their ultrastructure including subtle changes observed on prolonged culture are described. Other embryos showed some normal and obvious abnormal features, such as partial fragmentation and multinucleated blastomeres, or evidence of degeneration.
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References
Trounson A: Factors controlling normal embryo development and implantation of human oocytes fertilized in vitro.In Fertilization of the Human Egg in Vitro: Biological Basis and Clinical Applications, HM Beier, HR Lindner (eds). New York, Springer-Verlag, 1983, pp 235–250
Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fertil 1982;64: 285–294
Sathananthan AH, Wood C, Leeton J: Ultrastructural evaluation of 8–16 cell human embryos developed in vitro. Micron 1982;13:193–203
Mohr L, Trounson AO, Leeton JR, Wood C: Evaluation of normal and abnormal human embryo development during procedures in vitro.In Fertilization of the Human Egg in Vitro: Biological Basls and Clinical Application. HM Beier, HR Lindner (eds), New York, Springer-Verlag, 1983, pp 209–219
Trounson A, Conti A: Research in human in-vitro fertilization and embryo transfer. Br Med J 1982;285:244–248
Mahadevan MM, Trounson AO, Leeton JF. Relationship of tubal blockage, infertility of unknown cause, suspected infertility and endometriosis to success of in vitro fertilization and embryo transfer. Fertil Steril 1983;40:755–762
Sundstrom P, Nilsson O, Liedholm P: Cleavage rate and morphology of early human embryos obtained after artificial fertilization and culture. Acta Obstet Gynecol Scand 1981;60:109–120
Sathananthan AH, Trounson AO, Wood C, Leeton JF: Ultrastructural observations on the penetration of human sperm into the zona pellucida of the human egg in vitro. J Androl 1982;3:356–364
Sathananthan AH, Lopata A: Ultrastructure of human eggs. Aspirated preovulatory mature ova prior to fertilization. Micron 1980;11:469–470
Lopata A, Sathananthan AH, MaBain JC, Johnston WIH, Spiers AL: The ultrastructure of the preovulatory human egg fertilized in vitro. Fertil Steril 1980;33:12–20
Sathananthan AH: Ultrastructural morphology of fertilization and early cleavage in the human.In Basic-Science of in Vitro Fertilization and Embryo Transfer, C Wood, AO Trounson (eds). Edinburgh, Churchill Livingston 1984 (in press).
Willadsen SM: The developmental capacity of blastomeres from four and eight-cell sheep embryos. J Embryol Exp Morphol 1981;65:165–172
Trounson A, Mohr L: Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 1983;305:707–709
Dvorak M, Tesarik J, Pilka L, Travnik P: Fine structure of human two-cell ova fertilized and cleaved in vitro. Fertil Steril 1982;37:661–667
Lopata A, Kohlman D, Johnston I: Fine structure of normal and abnormal human embryos developed in culture.In Fertilization of the Human Egg in Vitro: Biological Basis and Clinical Applications, MM Beier, Lindner HR (eds). New York, Springer-Verlag, 1983, pp 189–210
Zamboni L, Mishell DR Jr, Bell JH, Baca M: Fine structure of the human ovum in the pronuclear stage. J Cell Biol 1966;30:579–600
Pereda J, Croxatto HB: Ultrastructure of a seven-cell human embryo. Biol Reprod 1978;18:481–489
Zamboni L, Thompson RS, Moore Smith D: Fine morphology of human oocyte maturation in vitro. Biol Reprod 1972;7:425–457
Sathananthan AH, Trounson AO: Ultrastrutural observations on cortical granules in human follicular oocytes cultured in vitro. Gam Res 1982;5:191–198
Soupart P, Strong PA: Ultrastructural observations on human oocytes fertilized in vitro. Fertil Steril 1974;25: 11–44
Tesarik J, Dvorak M: Ultrastructure of the human ovum fertilized in vitro. Folia Morphol (Prague) 1981;29:297–304
Dvorak M. The differentiation of rat ova during cleavage. Adv Anat Embryol Cell Biol 1978;55:1–131
Sathananthan AH, Trounson AO: Ultrastructure of cortical granule release and zona interaction in monospermic and polyspermic ova fertilized in vitro. Gam Res 1982;6:225–234
Austin CR: The Mammalian Egg. Oxford, Blackwell Scientific, 1961
Trounson A, Wood C: IVF results 1979–1982 at Monash University—Queen Victoria and Epworth Medical Centres. J Vitro Fert Embryo Transfer 1984;1:42–47
Angell RR, Aitken RJ, van Hook PFA, Lumsden MA, Templeton AA: Chromosome abnormalities in human embryos after in vitro fertilization. Nature 1983;303:336–338
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Trounson, A., Sathananthan, A.H. The application of electron microscopy in the evaluation of two- to four-cell human embryos cultured in vitro for embryo transfer. J Assist Reprod Genet 1, 153–165 (1984). https://doi.org/10.1007/BF01139208
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DOI: https://doi.org/10.1007/BF01139208