Abstract
The morphology of human, unfertilized oocytes and abnormal embryos cultured in vitro for 48–72 hr was examined in an attempt to learn more about oocyte maturation and reproductive failure in in vitro fertilization (IVF). About 21% of the unfertilized oocytes were totally degenerated. The majority (56%) of the remaining oocytes was arrested at the metaphase II stage. They contained coherent chromosomal plates and had extruded the first polar body with nuclear material. About 13% of oocytes underwent spontaneous activation. In most of these cases the second polar body was retained and many subnuclei or one big nucleus was formed. Five percent of metaphase II oocytes penetrated by sperm were not activated, likely as a result of oocyte immaturity. The developmental ability of abnormal embryos was poor. Seyeral one-cell-stage zygotes were arrested at the pronuclear stage or at mitosis of the first mitotic division. Poiyspermic embryos, especially those which contained four or more pronuclei, did not divide or formed uneven, multinucleated blastomeres. However, some triploid and tetraploid embryos often appeared normal morpholotisolty despite their lethal chromosomal abnormalities.
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References
Van Blerkom J: Developmental failure in human reproduction associated with preovulatory oogenesis and preimplantation embryogenesis.In Ultrastructure of Human Gametogenesis and Early Embryogenesis, J Van Blerkom, PM Motta (eds). Kluwer Academic Publishers, 1989, pp 125–180
Edwards RG: Causes of early embryonic loss in human pregnancy. Hum Reprod 1986;1:185–198
Sathananthan AH, Trounson AO: The human pronuclear ovum: Fine structure of monospermic and polyspermic fortilization in vitro. Gamete Res 1985;12:385–398
Van Blerkom J, Henry G: Cytogenetic analysis of living human eggs: cellular basis and developmental consequences of perturbations in chromosomal organization and complement. Hum Repord 1988;3:777–790
Ma S, Kalousek DK, Zouves C, Yuen BH, Gomel V, Moon YS: Chromosome analysis of human eggs failing to fertilize in vitro. Fertil Steril 1989;51:992–997
Gonen Y, Jacobson W, Casper RF: Gonadotropin suppresion with oral contraceptives before in vitro fertilization. Fertil Steril 1990;53:282–287
Quinn P, Warnes GM, Kerin JF, Kirby C: Culture factors in relation to the success of human in vitro fertilization and embryo transfer. Fertil Steril 1984;41:202–209
Veeck LL: Atlas of the Human Oocyte and Early Conceptus. Baltimore, Williams and Wilkims, 1986
Tarkowski AK: Development of single blastomeres.In Methods in Mammalian Embryology, JC Daniel (ed). San Francisco, Freeman, 1971, pp 172–185
Clarke HJ, Masui Y: Inhibition by dibutyryl cyclic AMP of the transition to metaphase of mouse oocyte nuclei and its reversal by cell fusion to metaphase oocytes. Dev Biol 1985;108:32–37
Tarkowski AK: An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 1966;4:394–400
Veeck LL, Wortham JWE, Witmeyer J, Sandow BA, Acosta AA, Garcia JA, Jones GS, Jones HW: Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 1983;39:594–602
Laufer N, Tarlatzis BC, de Cherney AH, Masters JT, Haseltine FP, MacLusky N, Naftolin F: Asynchrony between human cumulus-corona cell complex and oocyte maturation after human menopausal gonadotropin treatment for in vitro fertilization. Fertil Steril 1984;42:366–372
Sundstrom P, Nilsson BO: Metotic and cytoplasmic manration of oocytes collected in stimulated cycles is asynchranous. Hum Reprod 1988;3:613–619
Wassarman PM: The mammalian ovum:In The Physiology of Reprduction, E Knobil, J Neil et al. (eds), New York, Raven Press, 1988, pp 69–102
Edwards RG: Fertilization and chromosome anomalies in mammals.In Congenital Malformations. International Medical Congress, New York, 1964, pp 70–73
Soupart P, Strong PA: Ultrastructural obervations on polyspermic penetration of zona pellucida-free human oocytes inseminated in vitro. Fertil Steril 1975;26:523–537
Sathananthan AH, Trounson AO, Wood C: Atlas of Fine Structure of Human Sperm Penetration, Eggs and Embryos Cultured in Vitro. New York, Praeger Scientific; 1986
Yanagimachi R: Mammalian fertilization.In The Physiology and Reproduction, E Knobil, J Neil et al. (eds), New York, Raven Press, 1988, pp 135–185
Schmiady H, Kentenich H, Stauber M: Chromosome studies of human in vitro fertilization,In Cytogenetics, G Obe, A Basler (eds), Heidelberg, Springer-Verlag, Berlin, 1987, pp 184–197
Schmiady H, Kentenich H: Premature chromosome condensation after in vitro fertilization. Hum Reprod 1989;4:689–695
Clarke HJ, Masui Y: Transformation of sperm nuclei to metaphase chromosomes in the cytoplasm of maturing eggs of the mouse. J Cell Biol 1986;102:1039–1046
Thadani VM: Injection of sperm heads into immature rat oocytes. J Exp Zool 1979;210:161–168
O'Rand MG, Herman B, Digiuseppi J, Halme J, Hammond MG, Talbert LM: Analysis of deoxyribonucleic acid distribution in non-cleaving oocytes from patients undergoing in vitro fertilization. Fertil Steril 1986;46:452–460
Wramsby H, Fredga K: Chromosome analysis of human eggs failing to cleave after insemination in vitro. Hum Reprod 1987;2:137–142
Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination on in vitro fertilization and transfer of human embryos. J Reprod Fertil 1982;64:285–294
Sathananthan AH, Trounson AO: Ultrastructure of cortical granule release and zona interaction in monospermic and polyspermic human ova fertilized in vitro. Gamete Res 1982;6:225–234
Van Blerkom J, Henry GP, Porreco RP: Preimplantation human embryonic development from polynuclear eggs after in vitro fertilization. Fertil Steril 1984;41:686–696
Golbus MS, Bahraman R, Wiltse S, Hall BD: Tetraploidy in liveborn infant. J Med Genet 1976;13:329–332
Jacobs PA, Szulman AE, Funkhauser J, Matsuura JS, Wilson CC: Human triploidy: the relationship between the paternal origin of the additional haploid complement and development of partial hydatidiform mole. Ann Hum Genet 1982;46:223–231
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Bałakier, H., Casper, R.F. A morphologic study of unfertilized oocytes and abnormal embryos in human in vitro fertilization. J Assist Reprod Genet 8, 73–79 (1991). https://doi.org/10.1007/BF01138658
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DOI: https://doi.org/10.1007/BF01138658