Abstract
Ultrasonically guided follicular aspiration is now routinely used for in vitro fertilization (IVF) in many centers. We present an improved method for oocyte recovery. One hundred nine consecutive patients with mechanical infertility submitted for IVF were included in the study. Superovulation was induced with human menopausal gonadotropin (hMG)/human chorionic gonadotropin (hCG). Oocyte recovery was ultrasonically guided, the bladder filled with phosphate-buffered saline (PBS), and a cannula with trocar used for single percutaneous puncture. The aspiration needle was of 1.6 mm in internal diameter. When compared to published data using smaller-bore needles, it seems that this improved needle increased the number of oocytes recovered to a mean of 6.5 and the number of embryos replaced to a mean of 3.9 per patient, with a pregnancy rate of 20% per transfer.
Similar content being viewed by others
References
Lenz S, Lauritsen JG, Kjellow M: Collection of human oocytes for in-vitro fertilization by ultrasonically guided follicular puncture. Lancet 1981;1:1163
Lenz S, Lauritsen JG: Ultrasonically guided peratoneous aspiration of human follicles under local anesthesia: A new method of collecting oocytes for in-vitro fertilization. Fertil Steril 1982;38:673
Wikland M, Nilsson L, Hansson R, Hamberger L, Janson PO: Collection of human oocytes by the use of sonography. Fertil Steril 1983;39:603
Wikland M, Hamberger L: Ultrasound as a diagnostic and operative tool for in vitro fertilization and embryo replacement (IVF/ER) programs. J Vitro Fert Embryo Transfer 1984;1:213
Feichtinger W, Kemeter P: In-vitro fertilization and embryo transfer—an outpatient office procedure.In Recent Progress in Human in Vitro Fertilization, W Fiechtinger, P Kemeter (eds). Palermo, Cofese, 1984, p 285
Lewin A, Margalioth EJ, Rabinowitz R, Schenker JG: Comparative study of ultrasonically guided percutaneous aspiration with local anesthesia and laparoscopic aspiration of follicles in an in-vitro fertilization program. Am J Obstet Gynecol 1985;151:621
Lewin A, Laufer N, Rabinowitz R, Margalioth EJ, Bar I, Schenker JG: Ultrasonically guided oocyte collection under local anesthesia the first choice method for in-vitro fertilization—a comparative study with laparoscopy. Fertil Steril (in press)
Feichtinger W, Kemerter P: Laparoscopic or ultrasonically guided follicle aspiration for in vitro fertilization? J Vitro Fert Embryo Transfer 1984;1:244
DeCherney AH, Lavy G: Oocyte recovery methods in in-vitro fertilization. Clin Obstet Gynecol 1986;29:171
Margalioth EJ, Navot D, Laufer N, Mor-Yosef S, Rabinowitz R, Yarkoni S, Schenker JG: Zona-free hamster ovum penetration assay as a screening procedure for in-vitro fertilization. Fertil Steril 1983;40:386.
Laufer N, DeCherney AH, Haseltine FP, Polan ML, Mezer HC, Dlugi AM, Sweeney D, Nero F, Naftolin F: The use of high dose human menopausal gonadotropins in an in-vitro fertilization program. Fertil Steril 1983;40:734
Dulbecco R, Vogt M: Plaque formation and isolation of pure with poliomyelitis viruses. J Exp Med 1954;99:167–182
Lopata A, Johnston IW, Hoult JJ, Speirs AI: Pregnancy following intrauterine implantation of an embryo obtained by in-vitro fertilization of preovulatory eggs. Fertil Steril 1980;33:117
Lewin A, Laufer N, Rabinowitz R, Margalioth EJ, Bar I, Schenker JG: The effects of different isotonic solutions for bladder filling on the results of ultrasonically guided oocyte aspiration for IVF. Presented at the Fourth World Conference on in Vitro Fertilization, Melbourne, Australia, Nov. 18–22, 1985, Abstr 54
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lewin, A., Laufer, N., Rabinowitz, R. et al. Ultrasonically guided oocyte recovery for in vitro fertilization: An improved method. J Assist Reprod Genet 3, 370–373 (1986). https://doi.org/10.1007/BF01133250
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01133250