Abstract
Chromosomal abnormalities and abnormal embryonic development have previously been observed after human in vitro fertilization (IVF). Chromosomal abnormalities may arise not only after fertilization but even earlier during meiotic maturation of human oocytes in culture. Since chromosomal analysis is simple in oocytes during meiotic maturation, the chromosomal status was analyzed in oocytes which remained unfertilized in a human in vitro fertilization program. In 50 fertilization attempts the chromosomes of 62 unfertilized oocytes could be analyzed; 45 of them were in the process of meiotic maturation. In three oocytes two small polar bodies were observed 16–18 hr after insemination in the absence of fertilization. In one oocyte abnormal chromosome behavior was found during the first meiotic division, and in four oocytes during metaphase of the second meiotic division. These data suggest that chromosomal analysis of unfertilized oocytes in human IVF may improve the understanding of human oocyte maturation and fertilization.
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Lopata A, Kohlman D, Johnston I: The fine structure of normal and abnormal human embryos developed in culture.In Fertilization of the Human Egg in Vitro, HM Beier, HR Linder (eds). Berlin-New York, Springer Verlag, 1983, pp 189–210
Mohr LR, Trounson AO, Leeton JF, Wood C: Evaluation of normal and abnormal human embryo development during procedures in vitro.In Fertilization of the Human Egg in Vitro, HM Beier, HR Linder (eds). Berlin-New York Springer Verlag, 1983, pp 212–221
van Blerkom J, Henry G, Porreco R: Preimplantation human embryonic development from polynuclear eggs after in vitro fertilization. Fertil Steril 1984;41:686–696
Angell RR, Aitken RJ, van Look PFA, Lumbdsen MA, Templeton AA. Chromosomal abnormalities in human embryos after in vitro fertilization. Nature 1983;303:336–338
Rudak E, Dor J, Mashiach S, Nebel L., Goldamn B: Chromosome analysis of multipronuclear human oocytes fertilized in vitro. Fertil Steril 1984;41:538–545
Fujimoto S, Pahlavan N, Dukelow WR: Chromosome abnormalities in rabbit preimplantation blastocyst induced by superovulation. J Reprod Fertil 1974;40:177–181
Takagi N, Sasaki M: Digynic triploidy after superovulation in mice. Nature 1976;264:278–281
Asakawa T, Chan PJ, Dukelow WR: Time sequence of invitro maturation and chromosomal normality in metaphase I and metaphase II of the squirrel monkey (Samiri scureus) oocyte. Biol Reprod 1982;27:118–124
Maudlin I, Frazer LR: The effects of PMSG dose on the incidence of chromosomal anomalies in mouse embryos fertilized in vitro. J Reprod Fertil 1979;50:545–556
Edwards RG, Steptoe PC: Current status of in-vitro fertilization and implantation of human embryos. Lancet 1983;2:1265–1269
Tarkowski AK: An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 1966;5:394–400
Tsafriri A: Ooocyte maturation in mammals.In The Vertebrate Ovary, RE Jones (ed), New York, Plenum Press, 1978. pp 409–433
Ortiz ME, Salvatierra AM, Lopez J, Fernandez E, Croxatto HB: Postovulatory aging of human ova: Light microscopic observations. Gamete Res 1982;6:11–17
Ortiz ME, Lucero P, Croxatto HB: Postovulatory aging of human ova. II. Spontaneous dividion of the first polar body Gamete Res 1983;7:269–276
Wramsby H, Liedholm P: A gradual fixation method for chromosomal preparations of human oocytes. Fertil Steril 1984;41:736–738
Trapp M, Baukloh V, Bohneh HG, Heeschen W: Pollutants in human follicular fluid. Fertil Steril 1984;42:146–148
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Spielmann, H., Krüger, C., Stauber, M. et al. Abnormal chromosome behavior in human oocytes which remained unfertilized during human in vitro fertilization. J Assist Reprod Genet 2, 138–142 (1985). https://doi.org/10.1007/BF01131500
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DOI: https://doi.org/10.1007/BF01131500