Skip to main content
Log in

Culture of rabbit embryos and isolated blastomeres in hydrogel chambers implanted in the peritoneal cavity of intermediate mouse recipients

  • Published:
Journal of in Vitro Fertilization and Embryo Transfer Aims and scope Submit manuscript

Abstract

Chambers made from polymerized 2-hydroxyethyl methacrylate were used for the in vivo culture of rabbit embryos or isolated blastomeres. In Experiment 1, culture of 119, one-cell embryos for 72 hr in saline-filled chambers implanted in the peritoneal cavity of male mice resulted in 72 morulae and 25 blastocysts. Transfer of these 97 embryos to recipient does resulted in the birth of 3 live offspring (3%). Culture of 119, one-cell embryos for 72 hr in saline-filled chambers implanted in the peritoneal cavity of female mice resulted in 23 morulae and 68 blastocysts. Transfer of these 91 embryos to recipient does resulted in the birth of 20 live offspring (22%). All of the 119 control, one-cell embryos had degenerated after 72 hr of in vitro culture in saline-filled chambers. In Experiment 2, culture of 80 blastomeres isolated from four-cell embryos for 72 hr in compartmentalized, medium-filled chambers implanted in the peritoneal cavity of female mice resulted in 21 morulae and 43 blastocysts. For both experiments, the recovery rate for embryos or isolated blastomeres after in vivo culture was 100%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Whitten WK, Biggers JD: Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium. J Reprod Fert 1968;17:399–401

    Google Scholar 

  2. Bowen RA: Fertilization in vitro of feline ova by spermatozoa from the ductus deferens. Biol Reprod 1977;17:144–147

    Google Scholar 

  3. Kane MT, Foote RH: Culture of two- and four-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc Soc Exp Biol Med 1970;133:921–925

    Google Scholar 

  4. Maurer RR, Whitener RH, Foote RH: Relationship of in vivo gamete aging and exogenous hormones to early embryo development in rabbits. Proc Soc Exp Biol Med 1969; 131:882–885

    Google Scholar 

  5. Maurer RR, Onuma H, Foote RH: Viability of cultured and transferred rabbit embryos. J Reprod Fert 1970;21:417–422

    Google Scholar 

  6. Maurer RR: Advances in rabbit embryo culture.In Methods in Mammalian Reproduction, JC Daniel, Jr (ed). New York, Academic Press, 1978, pp 259–272

    Google Scholar 

  7. Whittingham DG, Biggers JD: Fallopian tube and early cleavage in the mouse. Nature 1967;213:942–943

    Google Scholar 

  8. Wright RW Jr, Bondioli KR: Aspects of in vitro fertilization and embryo culture in domestic animals. J Anim Sci 1981;53:702–729

    Google Scholar 

  9. Yanamigachi R, Chang MC: In vitro fertilization of golden hamster ova. J Exp Zool 1964;156:361–376

    Google Scholar 

  10. Whittingham DG, Bavister BD: Development of hamster eggs fertilized in vitro or in vivo. J Reprod Fert 1974;38:489–492

    Google Scholar 

  11. Winterberger S, Dauzier L, Thibault C: Le developpement in vitro de l'oeuf de la brebis et celui de la chevre. CR Acad Sci (Paris) 1953;147:1971–1973

    Google Scholar 

  12. Thibault C: La culture in vitro de l'oeuf de vache. Ann Biol Anim Biochem Biophys 1966;6:159–164

    Google Scholar 

  13. Lindner GM, Wright RW Jr: Morphological and quantitative aspects of the development of swine embryos in vitro. J Anim Sci 1978;46:711–718

    Google Scholar 

  14. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA: Normal development following in vitro fertilization in the cow. Biol Reprod 1982;27:147–158

    Google Scholar 

  15. Heyman Y, Menezo Y, Chesne P, Camous S, Garnier V: In vitro cleavage of bovine and ovine early embryos: Improved development using coculture with trophoblastic vesicles. Theriogenology 1987;27:59–68

    Google Scholar 

  16. Lutterbach A, Koll RA, Brem G: In vitro maturation of bovine oocytes in coculture with granulosa cells and their subsequent fertilization and development. Zuchthyg 1987; 22:145–150

    Google Scholar 

  17. Onanoff J: Recherches sur la fecondation et la gestation des mammiferes (Conclusions). CR Seanc Soc Biol (Paris) 1893;45:719

    Google Scholar 

  18. Fawcett DW, Wislocki GB, Waldo CM: The development of mouse ova in the anterior chamber of the eye and in the abdominal cavity. Am J Anat 1947;81:413–443

    Google Scholar 

  19. Briones H, Beatty RA: Interspecific transfers of rodent eggs. J Exp Zool 1954;125:99–118

    Google Scholar 

  20. Adams CE: The development of rabbit eggs in the ligated oviduct and their viability after re-transfer to recipient rabbits. J Embryol Exp Morphol 1973;29:133–144

    Google Scholar 

  21. De Mayo FJ, Mizoguchi H, Dukelow WR: Fertilization of squirrel monkey and hamster ova in the rabbit oviduct (xenogenous fertilization). Science 1980;208:1468–1469

    Google Scholar 

  22. Willadsen SM: Micromanipulation of embryos of the large domestic species.In Mammalian Egg Transfer, CE Adams (ed). Boca Raton, FL, CRC Press, 1982, pp 185–210

    Google Scholar 

  23. Boland MP: Use of the rabbit oviduct as a screening tool for the viability of mammalian eggs. Theriogenology 1984; 21:126–137

    Google Scholar 

  24. Sirard MA, Lambert RD, Menard DP, Bedoya M: Pregnancies after in-vitro fertilization of cow follicular oocytes, their incubation in rabbit oviduct and their transfer to the cow uterus. J Reprod Fert 1985;75:551–556

    Google Scholar 

  25. Sirard MA, Lambert RD: Birth of calves after in vitro fertilization using laparoscopy and rabbit oviduct incubation of zygotes. Vet Rec 1986;119:167–169

    Google Scholar 

  26. Moore NW, Adams CE, Rowson LEA: Developmental potential of single blastomeres of the rabbit egg. J Reprod Fert 1968;17:527–531

    Google Scholar 

  27. Willadsen SM: A method for culture of micromanipulated sheep embryos and its use to produce monozygotic twins. Nature 1979;277:298–300

    Google Scholar 

  28. Ratner BD: Biomedical application of hydrogels: Review and critical appraisal.In Biocompatibility of Clinical Implant Materials, DF Williams (ed). Boca Raton, FL, CRC Press, 1981, Vol II, pp 145–175

    Google Scholar 

  29. Pinchuk L, Eckstein EC: Pressurized polymerization for reaction casting of poly (2-hydroxyethyl methacrylate). J Biomed Mat Res 1981;15:183–189

    Google Scholar 

  30. Lee KH, Jee JG, Jhon MS, Ree T: Solute transport through crosslinked poly (2-hydroxyethyl methacrylate) membrane. J Bioeng 1978;2:269–278

    Google Scholar 

  31. Dulbecco R, Vogt M: Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med 1954;98:167–182

    Google Scholar 

  32. Steel RGD, Torrie JH: Principles and Procedures of Statistics. New York, McGraw-Hill, 1960

    Google Scholar 

  33. Lawson RAS, Adams CE, Rowson LEA: The development of sheep eggs in the rabbit oviduct and their viability after retransfer to ewes. J Reprod Fert 1972;29:105–116

    Google Scholar 

  34. Fisher B: Development retardation in cultured preimplantation rabbit embryos. J Reprod Fert 1987;79:115–123

    Google Scholar 

  35. Adams CE: The development of rabbit eggs after culture in vitro for 1–4 days. J Embryol Exp Morphol 1970;23:21–34

    Google Scholar 

  36. Yang X, Simkin M, Battista M, Wilcox B, Foote RH: Asynchronous embryo transfer in rabbits. Proc 12th Ann Conf Int Embryo Transfer Soc, Colorado Springs, Jan 12–14, 1986. Theriogenology 1986;25:219 (abstr)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This study represents a portion of a thesis submitted by the senior author in partial fulfillment of the requirements for the M.S. degree at Iowa State University.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pollard, J.W., Pineda, M.H. Culture of rabbit embryos and isolated blastomeres in hydrogel chambers implanted in the peritoneal cavity of intermediate mouse recipients. J Assist Reprod Genet 5, 207–215 (1988). https://doi.org/10.1007/BF01131124

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01131124

Key Words

Navigation