Abstract
Microinjection of capacitated sperm into the perivitelline space of oocyte was offered to one couple with persistent infertility of mixed origin. The husband's semen was sub-normal, whereas his wife had definitive tubal occlusion and polycystic ovaries. Four previous in vitro fertilization (IVF) attempts were performed but not fertilization was obtained. After superovulation, 13 oocytes were collected. Ten were submitted to microinjection and two were damaged during the procedure. One of the remaining eight had two pronuclei 18 hr after microinjection and progressed to a four-cell embryo after 48 hr. After reimplantation, a normal pregnancy was initiated, caryotype (46XX) was checked at 17 weeks. A normal and healthy girl has been delivered at term.
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Barwin BN: Intrauterine insemination of husband's semen. J Reprod Fertil 1974;36:101–106
Forrler A, Dellenbach P, Nisand I, Moreau L, Cranz C, Clavert A, Rumpler Y: Direct intraperitoneal insemination in unexplained and cervical infertility. Lancet 1986;1:916–917
Dellenbach P, Nisand I, Moreau L, Feger B, Plumere C, Gerlinger P, Brun B, Rumpler Y: Transvaginal sonographically controlled ovarian follicle puncture for egg retrieval. Lancet 1984;1:1467
Edwards RG, Steptoe PC, Purdy JM: Fertilization and cleavage in vitro of preovulatory human oocytes. Nature 1970;227:1307
Asch R, Ellsworth LR, Balmaceda JP, Wong PC: Pregnancy after translaparoscopic gamete intrafallopian transfer. Lancet 1984;2:1034
Menezo Y: Milieu synthétique pour la survie et la maturation des gamètes et pour la culture de l'oèuf fécondé. CR Acad Sci Paris 1976;282:1967–1970
Cohen J, Malter H, Wright G, Kort H, Massey J, Mitchell D: Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum Reprod 1989;4:435–442
Mathieu C, Guérin JF, Gille Y, Pinatel MC, Lornage J, Boulieu D: Séparation des spermatozoïdes en gradient de Percoll. J Gynecol Obstet Biol Reprod 1988;17:237–241
Ng SC, Bongso A, Sathanathan H, Ratnam SS: Micromanipulation: Its relevance to human in vitro fertilization. Fertil Steril 1990;53:203–219
Malter HE, Cohen J: Partial dissection of the human oocyte. A nontraumatic method using micromanipulation to assist zona pellucida penetration. Fertil Steril 1989;51:139–148
Ng SC, Bongso A, Ratnam SS, Sathanathan H, Chan CLK, Wong PC, Hagglund L, Anandakumar C, Wong YC, Goh WHH: Pregnancy after transfer of sperm under zona. Lancet 1988;2:790
Fishel S, Jackson P, Antinori S, Johnson J, Grossi S, Versaci C: Subzonal insemination for the alleviation of infertility. Fertil Steril 19XX;54:823–835
Salat-Baroux J, Alvarez S, Antoine JM, Cornet D, Plachot M, Mandelbaum J: Results of IVF in the treatment of polycystic ovary disease. Hum Reprod 1988;3:311–335
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Wittemer, C., Moreau, L., Dellenbach, P. et al. A case of human pregnancy after microinjection of capacitated sperm into the perivitelline space. J Assist Reprod Genet 8, 222–224 (1991). https://doi.org/10.1007/BF01130809
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DOI: https://doi.org/10.1007/BF01130809