Double transfer of embryos in in vitro fertilization, or is there a delayed receptivity of the endometrium?

  • Aby Lewin
  • Neri Laufer
  • Nili Yanay
  • Alex Simon
  • Efraim Zohav
  • Marius Berger
  • Joseph G. Schenker
Special Contributions


Sixty-seven in vitro fertilization (IVF) patients who underwent ultrasonically guided oocyte pickup (OPU) after induction of superovulation with human menopausal gonadotropins were submitted to two different protocols of embryo transfer. In the first group of 33 patients, four to five embryos were transferred to the uterus 48 hr after OPU. In the second group of 34 patients two to three embryos were transferred at 48 hr after OPU and two other embryos were transferred after freezing and thawing 48 hr later (96 hr after OPU). The pregnancy rate achieved in the double embryo transfer (ET) protocol was significantly higher than in the single-ET protocol (32.4 vs. 18.2%, respectively; P<0.05).

Key Words

in vitro fertilization embryo transfer 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cohen J: Obstetric outcome after assisted procreation —results of the french collaborative study. Presented at the 4th meeting of the European Society of Human Reproduction and Embryology, Barcelona, Spain, July 3–6, 1988Google Scholar
  2. 2.
    Lewin A, Laufer N, Rabinowitz R, Schenker JG: Ultrasonically guided oocyte recovery for in vitro fertilization: An improved method. J Vitro Fert Embryo Transfer 1986;3:370Google Scholar
  3. 3.
    Lassalle B, Testart J, Renard JP: Human embryo features that influence the success of cryopreservation with the use of 1,2-propanediol. Fertil Steril 1985:44:645PubMedGoogle Scholar
  4. 4.
    Croxatto HB, Ortiz ME, Diaz S, Hess R, Balmaceda, Croxatto HD: Studies on the duration of egg transport by the human oviduct. Am J Obstet Gynecol 1978;132:629PubMedGoogle Scholar
  5. 5.
    Croxatto HB, Diaz S, Fuentealba B, Croxatto HD, Carillo D, Fabres C: Studies on the duration of egg transport in the human oviduct. I. The time interval between ovulation and egg recovery from the uterus in normal women. Fertil Steril 1972;23:447PubMedGoogle Scholar
  6. 6.
    Diaz S, Ortiz ME, Croxatto HB: Studies on the duration of ovum transport by the human oviduct. III. Time interval between the luteinizing hormone peak and recovery of ova by transcervical flushing of the uterus in normal women. Am J Obstet Gynecol 1980;137:1161Google Scholar
  7. 7.
    Beatty RA: Transplantation of mouse eggs. Nature 1951; 168:2995Google Scholar
  8. 8.
    Newcomb R, Rowson LE: Conception rate after uterine transfer of cow eggs in relation to synchronization of oestrus and age of eggs. J Reprod Fertil 1975;43:539PubMedGoogle Scholar
  9. 9.
    Rowson LE, Moor RM: Embryo transfer in the sheep: The significance of synchronizing oestrus in the donor and recipient animal. J Reprod Fertil 1966;11:201Google Scholar
  10. 10.
    Lutjen PJ, Leeton JF, Findlay JK: Oocyte and embryo donation in IVF programs. Clin Obstet Gynecol 1985;12:799Google Scholar
  11. 11.
    Navot D, Laufer N, Kopolovic J, Rabinowitz R, Birkenfeld A, Lewin A, Granat M, Margalioth JE, Schenker JG: Artificially induced endometrial cycles and establishment of pregnancies in the absence of ovaries. N Engl J Med 1986;314:806PubMedGoogle Scholar
  12. 12.
    Rosenwaks Zev: Donor eggs: Their application in modern reproductive technologies. Fertil Steril 1987;47:895PubMedGoogle Scholar
  13. 13.
    Frydman R, Testart J, Giacomini P, Imbert MC, Martin E, Nahoul K: Hormonal and histological study of the luteal phase in women following aspiration of the preovulatory follicle. Fertil Steril 1982;38:312PubMedGoogle Scholar
  14. 14.
    Cohen J, Debache C, Pigeau F, Mandelbaum J, Plachot M, de Brux J: Sequential use of clomiphene citrate, human menopausal gonadotropin and human chorionic gonadotropin in human IVF. II. Study of luteal phase adequacy following aspiration of the preovulatory follicles. Fertil Steril 1984;42:360PubMedGoogle Scholar
  15. 15.
    0Abate V, Call A, Stanchi A: Endometrial biopsy at the time of embryo transfers: Correlation of histologic diagnosis with therapy and pregnancy rates. Fertil Steril 1984;41:43S (abstr)Google Scholar
  16. 16.
    Graf MJ, Reyniak JV, Battle-Mutter P, Laufer N: Histologic evaluation of luteal phase in woman following follicle aspiration for oocyte retrieval. Fertil Steril 1988;49:616PubMedGoogle Scholar
  17. 17.
    Salut-Baroux J, Giacomini P, Cornet D: Study of the luteal phase after ovulation and in vitro fertilization. Fertil Steril 1984;41:162 (abstr)Google Scholar
  18. 18.
    Mohr L, Trounson A, Freeman L: Deep-freezing and transfer of human embryos. J Vitro Fert Embryo Transfer 1985;2:1Google Scholar
  19. 19.
    Van Steirteghem AC, Van der Abbeel E: Cryopreservation of human embryos after GIFT and/or IVF. Presented at the joint meeting of the European Sterility Congress Organization and the European Society of Human Reproduction and Embryology, Budapest, Sept 27–30, 1987Google Scholar
  20. 20.
    Madjerek Z, De Visser J, Van der Vies J, et al.: Allylestrenol, a pregnancy maintaining oral Gestagen. Acta Endocrinol 1960;35:8–19PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Aby Lewin
    • 1
  • Neri Laufer
    • 1
  • Nili Yanay
    • 1
  • Alex Simon
    • 1
  • Efraim Zohav
    • 1
  • Marius Berger
    • 1
  • Joseph G. Schenker
    • 1
  1. 1.Department of Obstetrics and GynecologyHadassah-Hebrew University Medical SchoolJerusalemIsrael

Personalised recommendations