Abstract
Hyperprolactinemia, induced by pituitary isografts for 20 weeks in male mice and confirmed by radioimmunoassay using anti-mouse prolactin serum, did not impair spermatogenesis in the testis and maturing processes of spermatozoa in the epididymis. Incubation of freshly obtained epididymal spermatozoa for 90 min in culture media containing various levels of mouse prolactin did not yield any adverse effects on percentage motility rates of epididymal spermatozoa. When the level of mouse prolactin in the preincubation medium for epididymal spermatozoa was 100 ng/ml, the rate of fertilization by these preincubated spermatozoa in the subsequent in vitro fertilization experiment was significantly lowered compared with that observed in controls. However, when the level of prolactin in preincubation media was 10 ng/ml, no significant reduction in the rate of fertilization occurred. The present experiments seem to indicate the existence of some differences in the effects of prolactin on male germ cells until they reach the tail of the epididymis and on the processes of capacitation and/or fertilization by epididymal spermatozoa after they leave the epididymis.
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References
Fluckiger E, del Pozo E, von Werder K: Prolactin-Physiology, Pharmacology and Clinical Findings. Monographs on Endocrinology, Vo. 23. Berlin, Heidelberg, New York, Springer-Verlag, 1982.
Bartke A: Role of prolactin in reproduction in male mammals. Fed Proc 1980;39:2577–2581
Thorner MO, Rogol AD, Evans WS, Nunley WC, MacLeod RM: The effects of prolactin on gonadal function in man.In Central and Peripheral Regulation of Prolactin Function, RM MacLeod, U Scapagnini (eds), New York, Raven, 1980, pp 271–285
Franks S, Jacobs HS, Martin H, Nabarro JDN: Hyperprolactinaemia and impotence. Clin Endocrinol 1978;8:277–287
Segal S, Yaffe H, Laufer N, Ben-David M: Male hyperprolactinemia: Effects on fertility. Fertil Steril 1979;32:556–561
Svare B, Bartke A, Doherty P, Mason I, Michael SD, Smith MS: Hyperprolactinemia suppresses copulatory behavior in male rat and mice. Biol Reprod 1979;21:529–535
Nonomura M, Hoshino K, Harigaya T, Hashimoto H, Yoshida O: Effects of hyperprolactinaemia on reproduction in male mice. J Endocrinol 1985;107:71–76
Bartke A, Smith MS, Michael SD, Peron FG, Dalterio S: Effects of experimentally-induced chronic hyperprolactinemia on testosterone and gonadotropin levels in male rats and mice. Endocrinology 1977;100:182–187
Kasai K, Minato Y, Toyoda Y: Fertilization and development in vitro of mouse eggs from inbred strains and F1 hybrids. Jap J Anim Repro 1978;1:19–22
Mori C, Hoshino K: Cytofluorometry of nuclear DNA in ova and zygotes using mithramycin and DAPI during in vitro fertilization in mice. Acta Histochem Cytochem 1985;18:656
Harigaya T, Sakai S, Kohmoto K, Shoda Y: Influence of glucocorticoids on mammary prolactin receptors in pregnant mice after ovariectomy. J Endocrinol 1982;94:149–155
Johsen SG: Testicular biopsy score count—A method for registration of spermatogenesis in human testes: Normal values and results in 335 hypogonadal males. Hormones 1970;1:2–25
Hoshino K, Mori C: Comparative fluorocytophotometry of nuclear DNA in lymphocytes using acriflavin-Feulgen reaction and mithramycin. Acta Histochem Cytochem 1986;19:386
Kohmoto K: Mouse prolactin obtained by pituitary organ culture in a serum free medium. Endocrinol Jap 1975;22:465–469
Leemann U, Ruch F: Selective excitation of mithramycin or DAPI fluorescence on double-stained cell nuclei and chromosomes. Histochemistry 1978;58:329–334
Weiner RI, Bethea CL: Hypothalamic control of prolactin secretion.In Prolactin, RB Jaffe (ed). New York, Oxford, Elsevier, 1981, pp 19–55
Everett JW: Luteotrophic function of autografts of the rat hypophysis. Endocrinology 1954;54:685–690
Montemurro DG, Gardner WU: Effects of subcutaneous transplants of pituitary gland and hypothalamus on the estrous cycle of the mouse. Endocrinology 1963;73:174–184
Jequier AM, Crich JC, Ansell ID: Clinical findings and testicular histology. Fertil Steril 1979;31:525–530
Cunningham CR, Huchin C: FSH and prolactin-secreting pituitary tumors; Pituitary dynamics and testicular histology. J Clin Endocrinol Metab 1977;44:248–253
Hermanns U, Haffez ESE: Prolactin and male reproduction. Arch Androl 1981;6:95–125
Clausen OF, Abyholm T: Deoxyribonucleic acid flow cytometry of germ cells in the investigation of male infertility. Fertil Steril 1980;34:369–374
Fukuda A, Noda Y, Mori T, Mori C, Hashimoto H, Hoshino K: Effects of prolactin on gametes and zygotes during in vitro fertilization in mice. J Vitro Fert Embryo Transfer (in press)
Sheth AR, Mugatwala PP, Shah GV, Rao SS: Occurrence of prolactin in human semen. Fertil Steril 1975;26:905–907
Schoenfeld C, Amelar RD, Dubin L, Numeroff M: Prolactin, fructose, and zinc levels found in human seminal plasma. Fertil Steril 1979;32:206–208
Chan SYW, Tang LCH, Chan PH, Tang GWK, Ma HK: Relationships of seminal plasma prolactin with spermatozoal characteristics and fertilizing capacity in vitro. Arch Androl 1984;12:17–24
Smith ML, Luqman WA, Rakoff JS: Correlations between seminal radioimmunoreactive prolactin, sperm count, and sperm motility in prevasectomy and infertility clinic patients. Fertil Steril 1979;32:312–315
Ramirez AV, Rojas CV, Hicks JJ: Similar effects of prolactin and dbcAMP upon human spermatozoa metabolism. Int J Androl 1980;3:23–31
Shah GV, Desal RB, Sheth AR: Effect, of prolactin on metabolism of human spermatozoa. Fertil Steril 1976;27:1292–1294
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Mori, C., Hashimoto, H., Hoshino, K. et al. Influences of prolactin upon spermatogenesis and spermatozoa during in vitro fertilization in mice. J Assist Reprod Genet 5, 61–66 (1988). https://doi.org/10.1007/BF01130660
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DOI: https://doi.org/10.1007/BF01130660