Abstract
Between September 1987 and August 1989, all patients and their spouses entering our in vitro fertilization (IVF) program were screened for the human immunodeficiency virus (HIV) using the enzyme-linked immunosorbent assay (ELISA). Of 848 patients and 848 spouses tested, all but 4 patients and 1 husband tested negative. Of those who tested positive on repeat testing with ELISA, only one was positive on Western blotting (HIV prevalence, 0.59 per 1000). During this same time period 1187 samples of human cord blood were used to make tissue culture medium for the IVF embryology laboratory. One sample was discarded because of positive HIV on ELISA and Western blotting; two other samples were discarded because of positivity to the hepatitis B surface antigen. While we believe that routine HIV screening of IVF patients and their spouses is indicated, this population is of low risk for HIV positivity. Furthermore, continued screening of human sera used to make tissue culture media for IVF is mandatory.
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References
Centers for Disease Control: AIDS and human immunodeficiency virus infection in the United States Morbid Mortal Week Rep 1989;38:1–38
Burke DS, Brundage JF, Redfield RR, Damato JJ, Schable CA, Putman P, Visintine R, Kim HI: Measurement of the false positive rate in a screening program for human immunodeficiency virus infections. N. Engl. J Med 1988; 319:961–964
Centers for Disease Control: Transfusion-associated human T-lymphotropic virus type III/lymphadenopathy-associated virus infection from a seronegative donor—Colorado. Morbid Mortal Week Rep 1986;35:389–410
Ranki A, Krohn M, Allain J-P, Franchini G, Valle S-L, Antonen J, Leuther M, Krohn K: Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection. Lancet 1987;2:589–593
McDougal JS, Martin LS, Cort SP, Mozen M, Heidebrant CM, Evatt BL: Thermal inactivation of the acquired immunodeficiency syndrome, human T lymphotrophic virus-III/ lymphadenopathy-associated virus, with special reference to antihemophilic factor. J Clin Invest 1985;76:875–877
Resnick L, Veren K, Salahuddin SZ, Tondreau S, Markham PD: Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. JAMA 1986;255:1887–1891
Rutherford GW, Werdegar D: The epidemiology of acquired immunodeficiency syndrome.In AIDS-Pathogenesis and Treatment, JA Levy (ed). New York, Marcel Dekker, 1989, pp 1–36
Rogers MF: The epidemiology of pediatric HIV infection.In AIDS and Other Manifestations of HIV Infection, GP Wormser, RE Stahl, EJ Bottone (eds). Park Ridge, NJ, Noyes, 1987, pp. 42–47
Blanche S: A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. N Engl J Med 1989;320:1643–1648
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Edelstein, M.C., Kruithoff, C., Brothman, L. et al. The prevalence of human immunodeficiency virus in patients and their spouses entering a large in vitro fertilization program. J Assist Reprod Genet 7, 254–256 (1990). https://doi.org/10.1007/BF01129529
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DOI: https://doi.org/10.1007/BF01129529