The cellular β-galactosidase activities produced by thelac'Z gene ofEscherichia coli, cloned on YEp, YRp, or YCp-type plasmids in host cells ofSaccharomyces cerevisiae with different ploidies, and which was expressed by a modified jeastHIS5 promoter, showed characteristic differences depending on the plasmid. But for any given plasmid, the isogenic diploid and tetraploid transformants showed slightly lower enzyme activities than their respective haploid transformants. This was due to the similar copy numbers of the plasmids in host cells. Since the cell number per unit volume of the culture decreased with increasing cell ploidy, the enzyme activity per unit volume of the culture decreased significantly. The holding stability of plasmids increased with increasing ploidy of the host cell, especially that of the YRp plasmid. On the YRp plasmid, thelac'Z gene showed higher productivity withTRP1 thanLEU2 as the selection marker for the plasmid.
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Takagi, A., Chua, E.N., Boonchird, C. et al. Constant copy numbers of plasmids inSaccharomyces cerevisiae hosts with different ploidies. Appl Microbiol Biotechnol 23, 123–129 (1985). https://doi.org/10.1007/BF00938964
- Enzyme Activity
- Host Cell
- Unit Volume
- Selection Marker