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Amino Acids

, Volume 6, Issue 1, pp 107–110 | Cite as

PCR cloning of a protein-coding part of the thioredoxin gene fromStreptomyces aureofaciens

  • O. Labudová
  • M. Némethová
  • M. Kollárová
  • T. Skern
  • E. Küchler
Short Communication
  • 32 Downloads

Summary

The method of two-stage half-specific amplification was described and successfully used in the isolation of the protein-coding part of the thioredoxin gene from Streptomyces aureofaciens BMK. The efficiency of a new PCR modification for the specific amplification of the target DNA fragments (genes) with unknown sequences is compared with the used half-specific PCR. The determined target sequence demonstrates the highest homology with the thioredoxin genes from Corynebacterium nephridii C-1 and Anabaena 7119.

Keywords

Amino acids Half-specific PCR Thioredoxin Gene isolation Streptomyces aureofaciens 

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References

  1. Labudová O, Kollárová M (1991) Thioredoxin: structure, properties, fuctions. Biokhimiya 56: 977–989Google Scholar
  2. Lim C-J, Gleason FK, Futchs JA (1986) Cloning, expression and characterization of theAnabaena thioredoxin gene inE. coli. J Bacteriol 168: 1258–1264Google Scholar
  3. Meng M, Hogenkamp HPC (1981) Purification, characterization and amino acid sequence of thioredoxin fromCorynebacterium nephridii. J Biol Chem 256: 9174–9182Google Scholar
  4. Müller EGD, Buchanan BB (1989) Thioredoxin is essential for photosynthetic growth. J Biol Chem 164: 4008–4014Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • O. Labudová
    • 1
  • M. Némethová
    • 1
  • M. Kollárová
    • 1
  • T. Skern
    • 2
  • E. Küchler
    • 2
  1. 1.Department of BiochemistryFaculty of Science, Comenius UniversityBratislavaSlovakia
  2. 2.Department of BiochemistryMedical Faculty of UniversityViennaAustria

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