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Reverse action of hydrolases in frozen aqueous solutions

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Summary

The reverse action of hydrolases provides an attractive alternative to the chemical synthesis of peptides, oligosaccharides and oligonucleotides. Freezing the reaction mixture has proved to suppress competitive reactions in enzyme-catalysed peptide synthesis. After a short discussion of the influence of freezing on enzyme-catalysed reactions the current manuscript gives an overview of protease-catalysed peptide synthesis and the possible reasons of the yield-enhancing effect of freezing. The application of glycosidases and ribonucleases for synthetic purposes in frozen reaction mixtures is summarized.

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Abbreviations

Ac:

acetyl

Bz:

benzoyl

C>p:

cytidine 2′:3′-cyclic monophosphate

CpU:

cytidylyl (3′–5′)uridine

DTT:

dithioerythritol

Mal:

maleyl

NH2 :

amide

OBzl:

benzyl ester

OEt:

ethyl ester

OEt(Cl):

monochloroethyl ester

OMe:

methyl ester

pNA:

p-nitroanilide

Pht:

phthalyl

Z:

benzyloxycarbonyl

U:

uridine

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  1. Faculty of Biosciences, Pharmacy and Psychology, Institute of Biochemistry, Leipzig University, Talstrasse 33, D-04103, Leipzig, Germany

    M. Hänsler & H. -D. Jakubke

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  1. M. Hänsler
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  2. H. -D. Jakubke
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Hänsler, M., Jakubke, H.D. Reverse action of hydrolases in frozen aqueous solutions. Amino Acids 11, 379–395 (1996). https://doi.org/10.1007/BF00807943

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