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Free energies of amino acid adsorption on silica in neutral aqueous medium as estimated from high-performance liquid-chromatographic retention data

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Summary

Equilibrium constants (K) and free energies (−ΔG) of amino acid adsorption on silica in a neutral aqueous medium were calculated from the retention values measured by means of high-performance liquid chromatography on a silica gel column. For most amino acids (with the exception of proline) −ΔG values were negative andK < 1, thus showing very low adsorption. Influence of the structure of theα-substituent on adsorbability is analyzed. A linear dependence of −ΔG on the number of aliphatic carbon atoms was shown for the series: glycine-alanine-valine-leucine-isoleucine.

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Abbreviations

Gly:

glycine

Ala:

alanine

Pro:

proline

Val:

valine

Ile:

isoleucine

Leu:

leucine

Ser:

serine

Thr:

threonine

Cys:

cysteine

Asn:

asparagine

Gln:

glutamine

Asp:

aspartic acid

Glu:

glutamic acid

Met:

methionine

His:

histidine

Phe:

phenylalanine

Tyr:

tyrosine

DOPA:

3,4-dioxyphenylalanine

Trp:

tryptophan

References

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  • Pochapsky TC, Gopen Q (1992) A chromatographic approach to the determination of relative free energies of interaction between hydrophobic and amphiphilic amino acid side chains. Protein Sci 1: 786–795

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Authors and Affiliations

  1. Institute of Surface Chemistry, Academy of Sciences of the Ukraine, Kiev, Ukraine

    V. A. Basiuk & T. Yu. Gromovoy

  2. Instituto de Ciencias Nucleares, UNAM, Circuito Exterior C.U., A.Postal 70-543, 04510, Mexico, D.F., Mexico

    V. A. Basiuk

Authors
  1. V. A. Basiuk
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  2. T. Yu. Gromovoy
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Basiuk, V.A., Gromovoy, T.Y. Free energies of amino acid adsorption on silica in neutral aqueous medium as estimated from high-performance liquid-chromatographic retention data. Amino Acids 7, 305–309 (1994). https://doi.org/10.1007/BF00807705

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  • DOI: https://doi.org/10.1007/BF00807705

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