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Amino Acids

, Volume 7, Issue 3, pp 305–309 | Cite as

Free energies of amino acid adsorption on silica in neutral aqueous medium as estimated from high-performance liquid-chromatographic retention data

  • V. A. Basiuk
  • T. Yu. Gromovoy
Article
  • 82 Downloads

Summary

Equilibrium constants (K) and free energies (−ΔG) of amino acid adsorption on silica in a neutral aqueous medium were calculated from the retention values measured by means of high-performance liquid chromatography on a silica gel column. For most amino acids (with the exception of proline) −ΔG values were negative andK < 1, thus showing very low adsorption. Influence of the structure of theα-substituent on adsorbability is analyzed. A linear dependence of −ΔG on the number of aliphatic carbon atoms was shown for the series: glycine-alanine-valine-leucine-isoleucine.

Keywords

Amino acids Silica Adsorption Free energies Retention High-performance liquid chromatography 

Abbreviations

Gly

glycine

Ala

alanine

Pro

proline

Val

valine

Ile

isoleucine

Leu

leucine

Ser

serine

Thr

threonine

Cys

cysteine

Asn

asparagine

Gln

glutamine

Asp

aspartic acid

Glu

glutamic acid

Met

methionine

His

histidine

Phe

phenylalanine

Tyr

tyrosine

DOPA

3,4-dioxyphenylalanine

Trp

tryptophan

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References

  1. Greenland DJ, Laby RH, Quirk JP (1965a) Adsorption of amino acids and peptides by montmorillonite and illite. Part 1. Cation exchange and proton transfer. Trans Faraday Soc 61: 2013–2023Google Scholar
  2. Greenland DJ, Laby RH, Quirk JP (1965b) Adsorption of amino acids and peptides by montmorillonite and illite. Part 2. Physical adsorption. Trans Faraday Soc 61: 2024–2035Google Scholar
  3. Karger B (1971) Interrelation of theory and practice in high-speed liquid chromatography. In: Kirkland JJ (ed) Modern practice of liquid chromatography. John Wiley & Sons, New YorkGoogle Scholar
  4. Pochapsky TC, Gopen Q (1992) A chromatographic approach to the determination of relative free energies of interaction between hydrophobic and amphiphilic amino acid side chains. Protein Sci 1: 786–795Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • V. A. Basiuk
    • 1
    • 2
  • T. Yu. Gromovoy
    • 1
  1. 1.Institute of Surface ChemistryAcademy of Sciences of the UkraineKievUkraine
  2. 2.Instituto de Ciencias NuclearesUNAM, Circuito Exterior C.U.MexicoMexico

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