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An improved scheme of leucine derivative fragmentation in mass spectrometry

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Summary

This study illustrates the contribution of stable isotopes to amino acid mass spectrometry. The mass spectra of natural leucine and 113C leucine were compared and the mass fragmentography pattern analysed. This analysis indicates that the position of the stable isotope in tracer molecules should be very dependent on the analytical procedure used for their determination.

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References

  • Beaufrere B, Putet G, Pachiaudi C, Salle B (1990) Whole body protein turnover measured with13C leucine. Pediatr Res 28: 147–152

    Google Scholar 

  • Bier DM, Matthews DE (1982) Stable isotope tracer methods for in vivo investigations. Fed Proc 41: 2679–2685

    Google Scholar 

  • Carraro F, Rosenblatt J, Wolfe RR (1991) Isotopic determination of fibronectin synthesis in humans. Metabolism 40: 553–561

    Google Scholar 

  • Desgres J, Boisson D, Padieu P (1979) Gas liquid chromatography of isobutyl ester, N(O)-heptafluorobyturate derivatives of amino acids on a glass capillary column for quantitative separation in clinical biology. J Chromatogr 162: 133–152

    Google Scholar 

  • Halliday D, Read WWC (1981) Mass spectrometric assay of stable isotopic enrichment for the estimation of protein turnover in man. Proc Nutr Soc 40: 321–334

    Google Scholar 

  • McKenzie SL, Hogge LR (1977) Gas chromatography-mass spectrometry of the N(O) heptafluorobutyryl isobutyl esters of the protein amino acids using electron impact ionisation. J Chromatogr 132: 485–493

    Google Scholar 

  • Meguid MM, Matthews DE, Bier DM, Meredith CN, Soeldner JS, Young VR (1986) Leucine kinetics at graded leucine intakes in young men. Am J Clin Nutr 43: 770–780

    Google Scholar 

  • Pelletier V, Marks L, Wagner DA, Hoerr RA, Yong VR (1991) Branched chain amino acid interactions with reference to amino acid requirements in adult men: valine metabolism at different leucine intakes. Am J Clin Nutr 53:395–401

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biochimie, Faculté de Médecine, 15 rue A. Paré, F-42023, Saint Etienne Cédex 2, France

    C. Perier, J. Doumit & J. Frey

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  1. C. Perier
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  2. J. Doumit
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  3. J. Frey
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Perier, C., Doumit, J. & Frey, J. An improved scheme of leucine derivative fragmentation in mass spectrometry. Amino Acids 10, 273–276 (1996). https://doi.org/10.1007/BF00807329

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  • DOI: https://doi.org/10.1007/BF00807329

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