Summary
The effect of taurine on the Na+, K+, Cl− concentration and distribution in epithelial and compact layers of the human amniotic membrane had been investigated using the Bordeaux nuclear microprobe. Particle induced X-ray emission and Rutherford backscattering spectrometry techniques had been used to provice quantitative measurements. In physiological medium, the monovalent ions concentrations were identical in epithelial and compact layers. The addition of taurine in Hanks' physiological fluid had no effect on Na+ concentration, but decreased K+ and Cl− concentration in both layers. The quantitative results were related to electrophysiological observations on the effect of taurine on ionic exchanges through channels and paracellular pathways.
Similar content being viewed by others
References
Bara M (1976) Effets du magnésium sur des membranes liquides et bimoléculaires phospholipidiques. L'ion magnésium compétiteur des cations monovalents. Ann Biol Anim Bioch Biophys 16: 121–128
Bara M, Guiet-Bara A (1994) Inhibitor effects on the ionic exchanges through the human amniotic epithelial cell membranes. Cell Mol Biol 40: 1165–1171
Bara M, Guiet-Bara A, Durlach J (1985) Comparative study of the effects of magnesium and taurine on electrical parameters of natural and artificial membrane. Action on electrical constants of epithelial amniotic cell membranes. In: Halpern MJ, Durlach J (eds) Magnesium deficiency: physiopathology and treatment implications. Karger, Basel, pp 30–37
Bara M, Guiet-Bara A, Durlach J (1990) Comparative study of the effects of magnesium and taurine on electrical parameters of natural and artificial membranes. VII. Effects on cellular and paracellular ionic transfer through isolated human amnion. Magnesium Res 3: 249–254
Bara M, Guiet-Bara A, Moretto P, Razafindrabe L, Llabador Y, Simonoff M, Durlach J (1995) Nuclear microanalysis of the monovalent ions distribution in the human amnion. I. Effect of magnesium. Magnesium Res 8: 207–214
Doolittle LR (1985) Algorithms for the rapid stimulation of rutherford backscattering spectra. Nucl Instr Meth B9: 344–351
Durlach J (1988) Magnesium in clinical practice. J Libbey, London, p 360
Guiet-Bara A, Bara M, Durlach J (1991) Comparative study of the effects of magnesium and taurine on electrical parameters of natural and artificial membranes. VII. Effects on the ultrastructure of human amniotic epithelial cells. Magnesium Res 4: 35–39
Huxtable RJ (1992) Physiological actions of taurine. Physiol Rev 72: 101–163
Llabador Y, Bertault D, Gouillaud JC, Moretto Ph (1990) Advantages of high speed scanning for microprobe analysis of biological samples. Nucl Instr Meth B49: 435–440
Maxwell JA, Campbell JL, Teesdale WJ (1989) The Guelph PIXE software package. Nucl Instr Meth B43: 218–223
Moretto Ph, Razafindrabe L (1995) Simulation of RBS spectra for quantitative mapping of inhomogeneous biological tissue. Nucl Instr Meth (in press)
Moretto Ph, Llabador Y, Simonoff M, Razafindrabe L, Bara M, Guiet-Bara A (1993) Quantitative mapping of intracellular cations in human amniotic membrane. Nucl Instr Meth B77: 275–281
Moyer MS, Insler N, Dumaswala R (1992) The role of chloride in taurine transport across the human placental brush-border membrane. Bioch Biophys Acta 1109: 74–80
Pierno S, Tricarico D, Deluca A, Campagna F, Carotti A, Casini G, Camerino DC (1994) Effects of taurine analogues on chloride channel conductance in rat skeletal muscle fibers. A structure-activity relationship investigation. Naunyn Schmiedebergs Arch Pharmacol 349: 416–421
Razafindrabe L, Moretto P, Llabador Y, Simonoff M, Bara M, Guiet-Bara A (1995) Nuclear microanalysis of the human amnion: a study of ionic cellular exchanges. Nucl Instr Meth (in press)
Suleiman MS, Rodrigo GC, Chapman RA (1992) Interdependence of intracellular taurine and sodium in guinea-pig heart. Cardiovasc Res 26: 897–905
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guiet-Bara, A., Bara, M., Moretto, P. et al. Nuclear microanalysis of the monovalent ions distribution in the human amnion. Amino Acids 9, 353–362 (1995). https://doi.org/10.1007/BF00807272
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00807272