Summary
We studied the effect of recombinant human IL-1β (rhIL-1β) on hepatic amino acid (AA) flux in the isolated perfused rat liver model. Two experimental groups were used — a control group (n = 5) and a rhIL-1β-treated group (n = 5). IL-1 was added to the perfusate in two successive boluses of 0.1µg and 0.9µg, respectively 35 min (final concentration 0.67 ng/ml) and 60 min (6 ng/ml) after beginning the perfusion. In the IL-1 treated group, a reduction in flux was observed for only three AA, alanine, phenylalanine and serine. Glucose and urea production in the IL-1-treated group was slightly but not-significantly lower than in the controls.
rhIL-1β thus has only minor direct effects on AA flux and gluconeogenesis in the liver and cannot therefore be held responsible for the increase in hepatic amino acid uptake during stress.
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Lim, S.K., De Bandt, J.P., Ballet, F. et al. Effect of recombinant human interleukin 1β (rhIL-1β) on amino acid flux in the isolated perfused rat liver. Amino Acids 3, 139–146 (1992). https://doi.org/10.1007/BF00806779
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DOI: https://doi.org/10.1007/BF00806779