Skip to main content
Log in

Inhibition of sulfate excretion by (aminooxy)acetate induced stimulation of taurine excretion in rats

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary

l-Cysteine is mainly metabolized to sulfate and taurine through cysteinesulfinate pathway. Alternatively, sulfate is formed in rat liver mitochondria via 3-mercaptopyruvate pathway. Intraperitoneal administration of 5 mmol ofl-cysteine per kg of body weight resulted in the increase in sulfate and taurine (plus hypotaurine) excretion in the 24-h urine, which corresponded to 45.3 and 29.3%, respectively, ofl-cysteine administered. Subcutaneous injection of (aminooxy)acetate, a potent inhibitor of transaminases, together withl-cysteine halved the sulfate excretion and doubled the taurine excretion. In vitro sulfate formation froml-cysteine and froml-cysteinesulfinate in rat liver mitochondria was inhibited by (aminooxy)-acetate. The sulfate-forming activity of liver mitochondria obtained from rats injected with (aminooxy) acetate was also inhibited. These results indicate that the transamination reaction is crucial in sulfate formation and in the regulation of sulfur metabolism. Sulfur equilibrium in mammals was discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benjamin LE, Steele RD (1986) The effect of dietary protein on nitrogen and sulfur metabolism in portacaval-shunted rats. J Nutr 116: 59–69

    Google Scholar 

  • Cavallini D, De Marco C, Mondovi B (1958) Experiments withd-cysteine in the rat. J Biol Chem 230: 25–30

    Google Scholar 

  • Fellman JH, Roth ES (1985) The biological oxidation of hypotaurine to taurine: Hypotaurine as an antioxidant. In: Oja SS, Ahtee L, Kontro P, Paasonen MK (eds) Taurine. Biological actions and clinical perspectives. Alan R Liss, Inc., New York, pp 71–82

    Google Scholar 

  • Futani S, Ubuka T, Abe T (1994) High-performance liquid chromatographic determination of hypotaurine and taurine after conversion to 4-dimethylaminoazobenzene-4′-sulfonyl derivatives and its application to the urine of cysteine-administered rats. J Chromatogr B 660: 164–169

    Google Scholar 

  • Green TR, Fellman JK, Eicher, Pratt KL (1991) Antioxidant role and subcellular location of hypotaurine and taurine in human neutrophils. Biochim Biophys Acta 1073: 91–97

    Google Scholar 

  • Griffith OW (1983) Cysteinesulfinate metabolism. Altered partitioning between transamination and decarboxylation following administration ofβ-methyleneaspartate. J Biol Chem 258: 1591–1599

    Google Scholar 

  • Griffith OW (1987) Mammalian sulfur amino acid metabolism: an overview. Methods Enzymol 143: 366–376

    Google Scholar 

  • Hopper S, Segal HL (1964) Comparative properties of glutamic-alanine transaminase from several sources. Arch Biochem Biophys 105: 501–505

    Google Scholar 

  • Layne E (1957) Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3: 447–454

    Google Scholar 

  • Medes G (1937) CLXVII. Metabolism of sulphur. VI. Oxidation in the body of the sulphur-containing amino-acids and some of their partially oxidized derivatives. Biochem J 31: 1330–1346

    Google Scholar 

  • Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346–356

    Google Scholar 

  • Roy AB, Trudinger PA (1970) The biochemistry of inorganic compounds of sulphur. Cambridge University Press, Cambridge, pp 289–316

    Google Scholar 

  • Tanaka H, Nakatomi Y, Mori M, Ogura M (1990) Metabolism of methionine and cysteine in growing rats at various dietary protein levels. Agric Biol Chem 54: 2093–2099

    Google Scholar 

  • Tanaka H, Takahashi K, Ogura M (1993) Metabolic fate of cysteine sulfur in growing rats at various dietary protein levels. J Nutr Sci Vitaminol 39: 507–516

    Google Scholar 

  • Teraoka T, Ohta J, Abe T, Inoue H, Ubuka T (1993) Inhibition of sulfate-forming activity in rat liver mitochondria by (aminooxy)acetate. Amino Acids 5: 245–251

    Google Scholar 

  • Ubuka T, Kodama H, Mizuhara S (1967) Isolation of S-(carboxymethyl)cysteine from urine. Biochim Biophys Acta 141: 266–269

    Google Scholar 

  • Ubuka T, Abe T, Ohta J, Futani S, Fujiwara M (1993) In vivo and in vitro effect of (aminooxy)acetate on the cysteine metabolism in rats. Amino Acids 5: 154

    Google Scholar 

  • Ubuka T, Umemura S, Yuasa S, Kinuta M, Watanabe K (1978) Purification and characterization of mitochondrial cysteine aminotransferase from rat liver. Physiol Chem Phys 10: 483–500

    Google Scholar 

  • Ubuka T, Yuasa S, Ohta J, Masuoka N, Yao K, Kinuta M (1990) Formation of sulfate froml-cysteine in rat liver mitochondria. Acta Med Okayama 44: 55–64

    Google Scholar 

  • Ubuka T, Ohta J, Yao W-B, Abe T, Teraoka T, Kurozumi Y (1992)l-Cysteine metabolism via 3-mercaptopyruvate pathway and sulfate formation in rat liver mitochondria. Amino Acids 2: 143–155

    Google Scholar 

  • Wallach DP (1961) Studies on the GABA pathway — I The inhibition ofγ-aminobutyric acid —α-ketoglutaric acid transaminase in vitro by U-7524 (Amino-oxyacetic acid). Biochem Pharmacol 5: 323–331

    Google Scholar 

  • Yamada S, Abe T, Ohta J, Masuoka N, Ubuka T (1994) Increase in tissue cysteine level and excretion of sulfate and taurine after intragastric administration ofl-2-oxothiazolidine-4-carboxylate in rats. Amino Acids (in press)

  • Yoshida S, Akagi R, Ubuka T (1989) Sulfate and taurine excretion in rats afterl-cysteine administration. Acta Med Okayama 43: 281–288

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ubuka, T., Abe, T., Fujiwara, M. et al. Inhibition of sulfate excretion by (aminooxy)acetate induced stimulation of taurine excretion in rats. Amino Acids 8, 345–352 (1995). https://doi.org/10.1007/BF00806552

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00806552

Keywords

Navigation