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Electrophoretic polyamine transport in rat liver mitochondria

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Summary

Naturally occurring polyamines (spermidine, putrescine, cadaverine), as the well studied spermine, are transported into rat liver mitochondrial matrix provided that mitochondria are energized and the electrical membrane potential has a value of about 180 mV. This condition is achieved by the presence of inorganic phosphate, or acetate, or nigericin in the incubation medium. Valinomycin plus K+ almost completely blocks polyamine transport.

The obtained results clearly show that all naturally occurring polyamines are transported by an electrophoretic mechanism in responce to a high negative inner electrical potential.

The distribution ratio of polyamines across the mitochondrial membrane is far from the thermodynamic equilibrium by many orders of magnitude. This result might suggest the existence of a different pathway for polyamine efflux.

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Authors and Affiliations

  1. Centro Studio Fisiologia Mitocondriale del C.N.R. Padova, Dipartimento di Chimica Biologica, Universita' di Padova, Via Trieste 75, I-35121, Padova, Italy

    A. Toninello, L. Dalla Via, S. Testa & D. Siliprandi

Authors
  1. A. Toninello
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  2. L. Dalla Via
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  3. S. Testa
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  4. D. Siliprandi
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Toninello, A., Dalla Via, L., Testa, S. et al. Electrophoretic polyamine transport in rat liver mitochondria. Amino Acids 2, 69–76 (1992). https://doi.org/10.1007/BF00806076

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  • DOI: https://doi.org/10.1007/BF00806076

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