Summary
A 16-residue peptide was isolated from filtrate of uremic patients with carbohydrate intolerance by ultrafiltration with an Amicon Centriflo DM-5 membrane, followed by gel-filtration on Sephadex G-50 and Sephadex G-25, droplet countercurrent chromatography and high performance liquid chromatography. The hexadecapeptide thus obtained was identical as the entire amino acid sequence ofα-endorphin by amino acid analysis, application of the Edman degradation analysis and measurement of physical constants and analytical data of the synthetic hexadecapeptide. This result seems to suggest that an accumulation ofα-endorphin in uremic patients might cause carbohydrate intolerance.
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Abiko, T., Sekino, H. Isα-endorphin an uremic toxin?α-Endorphin isolated from filtrate of uremic patients with carbohydrate intolerance. Amino Acids 4, 35–43 (1993). https://doi.org/10.1007/BF00805799
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DOI: https://doi.org/10.1007/BF00805799