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A plant extract which enhances the plating efficiency of lymphoid cell lines and enhances the survival of normal lymphoid cells in vitro

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Abstract

A water soluble extract from the bark of the Samoan medicinal plantAlphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiencyin vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enhances the cloning efficiency of a T-hybridoma cell line by more than 30 times at otherwise unsuitably low serum concentrations, but does not completely substitute for serum. The enhanced growth of a B-cell hybridoma is also paralleled by an increased production of monoclonal antibodies in cultures containing low cell densities.

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Abbreviations

AZH/w:

water extract ofAlphitonia zizyphoides

AZH/c:

sugar-free fraction of the same extract

CM:

complete medium

FCS:

fetal calf serum

NMR:

nuclear magnetic resonance

PBS:

phosphate buffered saline

SEM:

standard error of the mean

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Department of Pharmacy, Division of Pharmacognosy, University of Uppsala, Biomedical Center, Uppsala, Sweden

    Christina Andersson Dunstan & Lars Bohlin

  2. Department of Immunology, University of Uppsala, Biomedical Center, Uppsala, Sweden

    Jan Andersson

  3. Department of Botany and Range Science, Brigham Young University, Provo, Utah, USA

    Paul A. Cox

  4. Section of Immunology and Cell Culture, National Veterinary Institute, BMC, Box 585, S-75123, Uppsala, Sweden

    Kjell-Olov Grönvik

Authors
  1. Christina Andersson Dunstan
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  2. Jan Andersson
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  3. Lars Bohlin
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  4. Paul A. Cox
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  5. Kjell-Olov Grönvik
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Andersson Dunstan, C., Andersson, J., Bohlin, L. et al. A plant extract which enhances the plating efficiency of lymphoid cell lines and enhances the survival of normal lymphoid cells in vitro. Cytotechnology 14, 27–38 (1994). https://doi.org/10.1007/BF00772193

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  • DOI: https://doi.org/10.1007/BF00772193

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