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Cytotechnology

, Volume 14, Issue 2, pp 81–87 | Cite as

Activation via TCR or IL-2 receptor of a CD8+ suppressor T cell clone: Effect on IL-10 production and on proliferation of the suppressor T cell

  • Yuji Minai
  • Tatsuhiro Hisatsune
  • Ken-Ichi Nishijima
  • Atsushi Enomoto
  • Shuichi Kaminogawa
Regular Research Papers

Abstract

In a previous study, we established CD8+ suppressor T cell (Ts) clone 13G2 which produced the suppressive lymphokine, interleukin-10 (IL-10). In this study, we examined what physiological activator could induce both production of IL-10 from 13G2 and the proliferation of 13G2. Both the antigenic stimulation mimicked by the anti-CD3 antibody and the T cell growth factor interleukin-2 (IL-2) induced IL-10 production from the 13G2 clone equally well. 13G2 cells proliferated remarkably with IL-2 stimulation, while anti-CD3 only slightly induced proliferation of the clone. 13G2 cells also produced IL-10 in the presence of hydroxyurea which blocked transit of cells from G1 to S phase. However, cycloheximide blocked the production of IL-10 from the Ts clone. The study demonstrates that both the anti-CD3 antibody and IL-2 induced IL-10 synthesis of the Ts clone equally well, and the proliferative response of Ts cells was induced more by IL-2 than by anti-CD3. IL-2 proved to be a good stimulator for Ts cells to produce suppressive lymphokine and to multiply their population.

CD8+ T cell IL-10 production proliferation 

Abbreviation

Ts

suppressor T cell

Th

helper T cell

Ag

antigen

APC

antigen presenting cell

IL

interleukin

TCR

T cell receptor

mAb

monoclonal antibody

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Yuji Minai
    • 1
  • Tatsuhiro Hisatsune
    • 1
  • Ken-Ichi Nishijima
    • 1
  • Atsushi Enomoto
    • 1
  • Shuichi Kaminogawa
    • 1
  1. 1.Department of Agricultural ChemistryUniversity of TokyoBunkyo-ku, TokyoJapan

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