Abstract
At the end of a hybridoma batch culture, the cells are usually discarded after separation from the culture broth. If, however, they are aseptically recycled into the reactor, the production process can be resumed simply by the addition of fresh medium. This cycle can then be repeated several times consecutively. In a test case, with a mouse hybridoma, we found antibody yields for each cycle in the same range as for a standard batch. In a 15 1 stirred tank reactor we could, within 6 days, produce 2.8 g of monoclonal antibody (MAb). This type of reactor operation allowed a doubling in the reactor volumetric productivity (mg/l/day).
Similar content being viewed by others
References
Feder J and Tolbert WR (June 1985) Mass culture of mammalian cells in perfusion systems. Intern. Biotechnol. Lab. 40–53.
Gramer MJ and Goochee CF (1992) Potential for degradation of glycoprotein oligosaccharides by extracellular glycosidases, Presentation at Cell Culture Engineering III, Feb 2–7, 1992, Palm Coast, Florida.
Griffiths JB (1988) Overview of cell culture systems and their scale-up. In: Spier RE and Griffiths JB (eds) Animal Cell Biotechnology (Vol 3, pp 179–220) London, Academic Press Ltd.
Mizrahi A (1988) Biologicals produced from animal cells in culture—an overview. Biotechnol Adv (Vol 6, pp 207–220). Pergamon Press.
Prior CP, Doyle KR, Duffy SA, Hope JA, Moellering JB, Prior GM, Scott RW and Tolbert WR (1989) The recovery of highly purified biopharmaceuticals from perfusion cell culture bioreactors. J. Parent. Sci. Technol. 43(1): 15–23.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blasey, H.D., Bernard, A.R. Repeated hybridoma batch culture with cell recycle. Cytotechnology 13, 51–53 (1993). https://doi.org/10.1007/BF00749975
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00749975