Abstract
The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFMI was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFMI, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.
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Abbreviations
- BSA:
-
bovine serum albumin
- CS:
-
calf serum
- DMEM:
-
Dulbecco's modified Eagle's medium
- ELISA:
-
enzyme-linked immunosorbant assay
- McAb:
-
monoclonal antibody
- PEG:
-
polyethylene glycol
- SFM:
-
serum-free medium
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Chen, Z., Ke, Y. & Chen, Y. A serum-free medium for hybridoma cell culture. Cytotechnology 11, 169–174 (1993). https://doi.org/10.1007/BF00749866
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DOI: https://doi.org/10.1007/BF00749866