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Production of monoclonal antibodies and ELISA for thebaine and codeine

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Abstract

The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Yukihiro Shoyama & Tomohiro Fukada

  2. Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-I Hakozaki, Higashi-ku, 812, Fukuoka, Japan

    Hiroki Murakami

Authors
  1. Yukihiro Shoyama
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  2. Tomohiro Fukada
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  3. Hiroki Murakami
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Shoyama, Y., Fukada, T. & Murakami, H. Production of monoclonal antibodies and ELISA for thebaine and codeine. Cytotechnology 19, 55–61 (1995). https://doi.org/10.1007/BF00749755

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  • DOI: https://doi.org/10.1007/BF00749755

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