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Cytotechnology

, Volume 11, Issue 1, pp 59–65 | Cite as

Effect of coexisting cells on the NGF-inducing neurite growth from PC12h-R

  • Hideaki Matsuoka
  • Yasushi Kazuno
  • Takuji Horie
  • Tomoo Homma
  • Yasuyuki Nemoto
Article

Abstract

Possible roles of coexisting cells in inducing neurite growth from a nerve cell were studied. Nerve growth factor (NGF)-inducing neurite growth from PC12h-R (a cell line derived from cultured nerve cells) was investigated at various cell densities. At the cell density 102∼104 cells/ml neurites appeared even without NGF. In contrast, no neurite appeared without NGF in single cell culture. The neurite growth observed in plural cell culture without NGF was only partially inhibited by antibody to NGF receptor (Ab-NGFR). However, the effect of the used medium alone was mostly inhibited by Ab-NGFR. These results suggest that the neurite inducing potency of coexisting cells is via different sites than the NGF receptor.

Key words

coexisting cell single cell neurite growth NGF PC12h-R 

Abbreviations

Ab-IgG-FITC

anti-mouse-IgG labeled with fluorescein isothiocyanate

Ab-NF

monoclonal antibody to neurofilament 160 kD

Ab-NGFR

monoclonal antibody to NGF receptor

BDNF

brain-derived neurotrophic factor

D-medium

medium for differentiation culture

DMEM

Dulbecco's modified Eagle's medium

M-medium

medium for multiplication culture

NGF

nerve growth factor

NGFR

NGF receptor

NT-3

neurotrophin-3

PC12

pheochromocytoma cell line

PC12h-R

subclone of PC12

Sup-D

supernatant of D-medium

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Hideaki Matsuoka
    • 1
  • Yasushi Kazuno
    • 1
  • Takuji Horie
    • 1
  • Tomoo Homma
    • 1
  • Yasuyuki Nemoto
    • 1
  1. 1.Department of Biotechnology, Faculty of TechnologyTokyo University of Agriculture and TechnologyKoganei, TokyoJapan

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