, Volume 11, Issue 1, pp 59–65 | Cite as

Effect of coexisting cells on the NGF-inducing neurite growth from PC12h-R

  • Hideaki Matsuoka
  • Yasushi Kazuno
  • Takuji Horie
  • Tomoo Homma
  • Yasuyuki Nemoto


Possible roles of coexisting cells in inducing neurite growth from a nerve cell were studied. Nerve growth factor (NGF)-inducing neurite growth from PC12h-R (a cell line derived from cultured nerve cells) was investigated at various cell densities. At the cell density 102∼104 cells/ml neurites appeared even without NGF. In contrast, no neurite appeared without NGF in single cell culture. The neurite growth observed in plural cell culture without NGF was only partially inhibited by antibody to NGF receptor (Ab-NGFR). However, the effect of the used medium alone was mostly inhibited by Ab-NGFR. These results suggest that the neurite inducing potency of coexisting cells is via different sites than the NGF receptor.

Key words

coexisting cell single cell neurite growth NGF PC12h-R 



anti-mouse-IgG labeled with fluorescein isothiocyanate


monoclonal antibody to neurofilament 160 kD


monoclonal antibody to NGF receptor


brain-derived neurotrophic factor


medium for differentiation culture


Dulbecco's modified Eagle's medium


medium for multiplication culture


nerve growth factor


NGF receptor




pheochromocytoma cell line


subclone of PC12


supernatant of D-medium


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Hideaki Matsuoka
    • 1
  • Yasushi Kazuno
    • 1
  • Takuji Horie
    • 1
  • Tomoo Homma
    • 1
  • Yasuyuki Nemoto
    • 1
  1. 1.Department of Biotechnology, Faculty of TechnologyTokyo University of Agriculture and TechnologyKoganei, TokyoJapan

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