Abstract
Possible roles of coexisting cells in inducing neurite growth from a nerve cell were studied. Nerve growth factor (NGF)-inducing neurite growth from PC12h-R (a cell line derived from cultured nerve cells) was investigated at various cell densities. At the cell density 102∼104 cells/ml neurites appeared even without NGF. In contrast, no neurite appeared without NGF in single cell culture. The neurite growth observed in plural cell culture without NGF was only partially inhibited by antibody to NGF receptor (Ab-NGFR). However, the effect of the used medium alone was mostly inhibited by Ab-NGFR. These results suggest that the neurite inducing potency of coexisting cells is via different sites than the NGF receptor.
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Abbreviations
- Ab-IgG-FITC:
-
anti-mouse-IgG labeled with fluorescein isothiocyanate
- Ab-NF:
-
monoclonal antibody to neurofilament 160 kD
- Ab-NGFR:
-
monoclonal antibody to NGF receptor
- BDNF:
-
brain-derived neurotrophic factor
- D-medium:
-
medium for differentiation culture
- DMEM:
-
Dulbecco's modified Eagle's medium
- M-medium:
-
medium for multiplication culture
- NGF:
-
nerve growth factor
- NGFR:
-
NGF receptor
- NT-3:
-
neurotrophin-3
- PC12:
-
pheochromocytoma cell line
- PC12h-R:
-
subclone of PC12
- Sup-D:
-
supernatant of D-medium
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Matsuoka, H., Kazuno, Y., Horie, T. et al. Effect of coexisting cells on the NGF-inducing neurite growth from PC12h-R. Cytotechnology 11, 59–65 (1993). https://doi.org/10.1007/BF00749058
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DOI: https://doi.org/10.1007/BF00749058