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Cytotechnology

, Volume 11, Issue 1, pp 35–40 | Cite as

A new type porous carrier and its application to culture of suspension cells

  • Takehiko Gotoh
  • Hiroyuki Honda
  • Naohiro Shiragami
  • Hajime Unno
Article

Abstract

A new type porous carrier was fabricated from a mixture of sodium alginate, bovine serum albumin and sodium bicarbonate. The porous space of the carrier is an assembly of void spaces. The carrier was successfully applied to the cultivation of suspension animal cells. In the culture, while both cells and carriers were held in suspension, the cells were entrapped hydrodynamically into the void spaces in the carriers. A culture of hybridoma cells using this carrier resulted in a cell density up to 5.7×107 cells per ml-carrier.

Key words

porous carrier suspension-cell culture 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Takehiko Gotoh
    • 1
  • Hiroyuki Honda
    • 1
  • Naohiro Shiragami
    • 1
  • Hajime Unno
    • 1
  1. 1.Department of BioengineeringTokyo Institute of TechnologyYokohamaJapan

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