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Cytotechnology

, Volume 5, Supplement 1, pp 59–60 | Cite as

Characterization of a subfamily of zinc finger genes expressed in human hematopoietic cell lines

  • M. Munaro
  • D. Petroni
  • M. Di Fazio
  • P. Comi
  • S. Ottolenghi
Selected Paper
  • 29 Downloads

Abstract

We isolated by low strigency screening of a human erythroleukemia cDNA library (K562) 45 indipendent clones hybridizing to a Kruppel-like (HF.10) zinc finger cDNA. The expression of 15 such cDNAs in human hematopoietic cell lines was investigated. Preliminary sequence analysis of the zinc finger motifs in these cDNAs indicate that they belong to a subclass of the Cys-Cys/His-His motif, showing the highest homology to the Wilm's tumor and EGR1, EGR2 cDNAs.

Keywords

Zinc Sequence Analysis cDNA Library Zinc Finger Hematopoietic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    H.J.Thiesen (1990) “Multiple Genes Encoding Zinc Finger Domains Are Expressed In Human T Cells” The New Biologist 2, 363–374.Google Scholar
  2. 2.
    K.Struhl (1989) “Helix-turn-helix, zinc finger and leucine zipper motifs for eukaryotic transcriptional regulatory proteins”, TIBS 14, 137–140.Google Scholar
  3. 3.
    A.Pannuti, L.Lanfrancone, A.Pascucci, P.G.Pelicci, G.LaMantia L.Lania (1988) “Isolation of cDNAs encoding finger proteins and measurement of the corresponding mRNA levels during myeloid terminal differentiation” Nucleic Acids Research 10, 4227–4237.Google Scholar
  4. 4.
    K.M.Call, T.Glaser, C.Y.Ito, A.J.Buckler et al. (1990) “Isolation and Characterization of a Zinc Finger Polypeptide Gene at the Human Chromosome 11 Wilm's Tumor Locus”, Cell 60, 509–520.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • M. Munaro
    • 1
  • D. Petroni
    • 3
  • M. Di Fazio
    • 1
  • P. Comi
    • 3
    • 2
  • S. Ottolenghi
    • 3
    • 1
  1. 1.Dipartimento di Genetica e Biologia dei MicrorganismiCNRMilanoItaly
  2. 2.Dipartimento di Scienze e Tecnologie BiomedicheCNRMilanoItaly
  3. 3.Centro di Studi sulla Patologia CellulareCNRMilanoItaly

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