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Cytotechnology

, Volume 5, Supplement 1, pp 51–54 | Cite as

Alternative methods in toxicology tests: In vitro toxicity

  • S. Cinelli
  • A. Falezza
  • C. Meli
  • P. Ciliutti
  • J. A. Vericat
Selected Paper

Abstract

Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develope alternative methods to reduce the required number of animals. The newinvitro assays offer numerous advantages such as speed, reproducibility and control of test conditions, and increased sensitivity. Although the dermal irritation assays might be substituted by theinvitro tests in the near future (Duffy, 1989), much work is required to evaluate organ toxicity withinvitro methods. We present data regarding the use of Balb/3T3 mice fibroblasts and primary rat hepatocytes as test systems forinvitro toxicity. The end-points we have analysed are total protein content, dye accumulation in lysosomes, reductase mytochondrial activity, intracellular content and leakage of enzymes into the medium.

Keywords

Enzyme Toxicity Protein Content Total Protein Toxicology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • S. Cinelli
    • 1
  • A. Falezza
    • 1
  • C. Meli
    • 1
  • P. Ciliutti
    • 1
  • J. A. Vericat
    • 1
  1. 1.Department of Genetic and Cellular ToxicologyLife Science Research-Roma Toxicology Centre, S. p. A.Pomezia(Roma)Italy

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