Abstract
The expression of β4, α6, and β1 integrin subunits has been investigated on somein vitro andin vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate thata) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.
Similar content being viewed by others
References
Kennel S. J., Lankford P. F., Foote L. J., Tsakeres F. S., Adams L. M. and Ullrich R. L. “Distribution of a tumor surface protein common to several murine lung carcinomas”. Cancer Res. 40: 2153–2159, 1980.
Falcioni R., Kennel S. J., Giacomini P., Zupi G. and Sacchi A. “Expression of TSP-180 correlates with metastatic potential of 3LL and B16 clones in mice” Cancer Res. 46: 5772–5778, 1986.
Kennel S. J., Foote L. J., Falcioni R., Sonnenberg A., Crouse C. and Hemler M. E. “Analysis of TSP-180: identity with α6/ß4 in the integrin superfamily” J. Biol. Chem. 264: 15515–15521, 1989.
Hynes R. O. “Integrins: a family of cell surface receptors”: Cell 48: 549–554, 1987.
Hemler M. E., Crouse C., Takada Y. and Sonnenberg A. “Multiple vary late antigen heterodimers on platelets”. J. Biol. Chem. 263: 7660–7665, 1988.
Sonnenberg A., Modderman P. W., Hogervorst E. “Laminin receptor on platelets is the integrin VLA-6” Nature 336: 487–489, 1989.
Hemler M. E., Crouse C. and Sonnenberg A. “Association of the VLA-α6 subunit with a novel protein” J. Biol: Chem: 264: 6529–6535, 1989.
Kajiji S., Tamura R. N. and Quaranta V. “A novel integrin suggests a fourth family of integrin adhesion receptors”. Embo J. 8: 673–680, 1989.
Zupi G., Cavallari A., Greco C. and Sacchi A. “Lewis lung carcinoma lines with different metastatic behavior: response to ICRF-159” Anticancer Res. 4: 91–95, 1984.
Falcioni R, Sacchi A., Reseau J. and Kennel S. J. “Mabs to human carcinoma associated protein complex”. Cancer Res. 49: 2615–2620, 1989.
Suzuki S. and Naitoh Y. “Amino acid sequence of a novel integrin ß4 subunit and expression of the mRNA in epithelial cells” Embo J. 9: 757–763, 1990.
Falcioni R., Perrotti N., Piaggio G., Kennel S. J. and Sacchi A. “Insulin induced phosphorylation of the ß4 subunit”. Molecular Carcinogenesis 2: 361–368, 1989.
Mariani-Costantini R., Falcioni R., Battista P., Zupi G., Kennel S. J. and Sacchi A. “Integrin α6/ß4 expression in human lun cancer as monitored by specific monoclonal antibodies” Cancer Res. in press, 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cimino, L., Perrotti, D., Falcioni, R. et al. \4 integrin expression onin vitro andin vivo metastatic variants of lewis lung carcinoma. Cytotechnology 5 (Suppl 1), 45–48 (1991). https://doi.org/10.1007/BF00736805
Issue Date:
DOI: https://doi.org/10.1007/BF00736805