, Volume 5, Supplement 1, pp 41–44 | Cite as

Expression of anchorin CII mRNA by cultured chondrocytes

  • Patrizio Castagnola
  • Ranieri Cancedda
Invited Lecture


The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during “in vitro” chondrocyte differentiation, little is known about the expression of the gene coding for its receptor: anchorin CII. The modulation of the anchorin mRNA steady state level in chick embryo chondrocytes at different developmental stages is described here.

The anchorin mRNA level was low in dedifferentiated chondrocytes, progressively increased after the cell transfer into suspension (a condition promoting differentiation), reached its maximal value after 4 weeks and decreased after 5 weeks.

Therefore anchorin CII mRNA reaches its maximum level in hypertrophic stage II chondrocytes.


Steady State mRNA Level Developmental Stage Culture System Maximum Level 
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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Patrizio Castagnola
    • 1
  • Ranieri Cancedda
    • 1
  1. 1.Istituto Nazionale per la Ricerca sul CancroGenovaItaly

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