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Cytotechnology

, Volume 5, Supplement 1, pp 3–6 | Cite as

Evaluation of the antiviral effect of new compounds by using cellular cultures

  • F. Dianzani
  • G. Antonelli
Invited Lecture
  • 19 Downloads

Abstract

Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceeded that detectable in the medium. In these conditions the replication curve of HIV is completed at 24 h post infection. At lower multiplicity of infection, virus yield peaks at approximately 72 h post-infection, and the virus released in the medium is negligible with respect to that which remains cell-associated. The method based on back-titration of virus in cryolysates of C8166 cells infected with HIV and treated with antiviral compounds has been used to evaluate HIV sensitivity to some agents. This method allows a precise and quick determination of the degree of activity of antiviral drugs.

Keywords

C8166 Cell Post Infection Antiviral Drug Infectious Virus Cycle Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • F. Dianzani
    • 1
  • G. Antonelli
    • 1
  1. 1.Institute of VirologyRomeItaly

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