Abstract
Production of 7 fatty acids and 13 amino acids of phytoplankton from Kinu-ura Bay, Japan, was determined in 1984 by 24-h time-series experiments, using a combined13C and gas chromatography/mass spectrometry (GC/MS) method. The data were compared with the net increase in carbon of these compounds with time, and the specific production of these organic components was calculated. Production of total amino acids attained a maximum rate between 9:00 and 12:00 hrs, while a decrease in this rate was observed between 12:00 and 15:00 hrs, when production rate of total fatty acids was maximum. These changes appeared to be caused by the nitrogen deficiency which occurred during this period. Increase in the relative proportion of 16:0 and 16:1 fatty acids to total fatty acids was observed, while decrease of 18:2 and 18:3 fatty acids in the photosynthetic products was noted at 12:00 hrs. No systematic diel change was observed in the amino acid composition of photosynthetic products throughout the incubation period. The specific production rates of fatty acids and amino acids were less than those of water-extractable carbohydrates even after day-night incubation. Such differences were most likely due to nitrogen deficiency in phytoplankton cells. Photosynthetic production of each organic compound estimated by the combined13C and GC/MS method generally accounted for ca. 70 to 80% of its net increase in organic carbon. Other sources of carbon were assumed to be related to the net increase in these compounds.
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Antia, N. J., McAllister, C. D., Parsons, T. R., Stephens, K., Strickland, J. D. H. (1963). Further measurements of primary production using a large-volume plastic sphere. Limnol. Oceanogr. 8: 166–183
Barlow, R. G. (1982a). Phytoplankton ecology in the Southern Benguela current. II. Carbon assimilation patterns. J. exp. mar. Biol. Ecol. 63: 229–237
Barlow, R. G. (1982b). Phytoplankton ecology in the Southern Benguela current. III. Dynamics of a bloom. J. exp. mar. Biol. Ecol. 63: 239–248
Barlow, R. G. (1984a). Physiological responses of phytoplankton to turbulent and stable environments in an upwelling region. J. Plankton Res. 6: 385–397
Barlow, R. G. (1984b). Time-series uptake of carbon into photosynthetic products of Benguela phytoplankton populations. J. Plankton Res. 6: 435–442
Ben-Amotz, A., Tornabene T. G., Thomas, W. H. (1985). Chemical profile of selected species of microalgae with emphasis on lipids. J. Phycol. 21: 72–81
Cuhel, R. L., Ortner, P. B., Lean, D. R. S. (1984). Night synthesis of protein by algae. Limnol. Oceanogr. 29: 731–744
Eppley, R. W. (1981). Relations between nutrient assimilation and growth in phytoplankton with a brief review of estimates of growth rate in the ocean. In: Platt, T. (ed.) Physiological bases of phytoplankton ecology. Can. Bull. Fish. aquat. Sciences 210: 251–263
Eppley, R. W., Sloan, P. R. (1965). Carbon balance experiments with marine phytoplankton. J. Fish. Res. Bd Can. 22: 1083–1097
Gieskes, W. W., Kraay, G. W. (1984). State-of-the-art in the measurement of primary production. In: Fasham, M. J. R. (ed.) Flows of energy and materials in marine ecosystems, theory and practice. Plenum Press, New York, p. 171–190
Hama, J., Handa, N. (1992). Diel photosynthetic production of cellular organic matter in natural phytoplankton populations, measured with13C and gas chromatography/mass spectrometry. I. Monosaccharides. Mar. Biol. 112: 175–181
Hama, T. (1988).13C-GC-MS analysis of photosynthetic products of the phytoplankton population in the regional upwelling area around the Izu Islands, Japan. Deep-Sea Res 35: 91–110
Hama, T., Handa, N. (1987). Pattern of organic matter production by natural phytoplankton population in a eutrophic lake. 1. Intracellular products. Arch. Hydrobiol. 109: 107–120
Hama, T., Handa N., Hama, J. (1987). Determination of amino acid production rate of a marine phytoplankton population with13C and gas chromatography-mass spectrometry. Limnol. Oceanogr. 32: 1144–1153
Hama, T., Handa, N., Takahashi, M., Whitney, F., Wong, C. S. (1988a). Change in distribution patterns of photosynthetically incorporated C during phytoplankton bloom in controlled experimental ecosystem. J. exp. mar. Biol. Ecol. 120: 39–56
Hama, T., Honjo, T. (1987). Photosynthetic products and nutrient availability in phytoplankton population from Gokasho Bay, Japan. J. exp. mar. Biol. Ecol. 112: 251–266
Hama, T., Matsunaga, K., Handa, N., Takahashi, M. (1988b). Day-night changes in production of carbohydrate and protein by natural phytoplankton population from Lake Biwa, Japan. J. Plankton Res. 10: 941–955
Hama, T., Matsunaga, K., Handa, N., Takahashi, M. (1990). Composition of photosynthetic products in Lake Biwa, Japan; vertical and seasonal changes and their relation to environmental factors. J. Plankton Res. 12: 133–147
Hama, T., Miyazaki, T., Ogawa, Y., Iwakuma, T., Takahashi, M., Otsuki, A., Ichimura, S. (1983). Measurement of photosynthetic production of a marine phytoplankton population using a stable13C isotope. Mar. Biol. 73: 31–36
Kaiser, F. E., Gehrke, C. W., Zumwalt, R. W., Kuo, K. C. (1974). Amino acid analysis: hydrolysis, ion-exchange cleanup, derivatization, and quantitation by gas-liquid chromatography. J. Chromat. 94: 113–133
Kouchi, H. (1982). Direct analysis of13C abundance in plant carbohydrates by gas chromatography-mass spectrometry. J. Chromat. 241: 305–323
Lancelot, C., Mathot, S. (1985a). Biochemical fractionation of primary production by phytoplankton in Belgian coastal waters during short- and long-term incubations with14C-bicarbonate. I. Mixed diatom population. Mar. Biol. 86: 219–226
Lancelot, C., Mathot, S. (1985b) Biochemical fractionation of primary production by phytoplankton in Belgian coastal waters during short- and long-term incubations with14C-bicarbonate II. Phaeocystis poucheti colonial population. Mar. Biol. 86: 227–232
Li, W. K. W., Glover, H. E., Morris, I. (1980). Physiology of carbon photoassimilation by Oscillatoria thiebautii in the Caribbean Sea. Limnol. Oceanogr. 25: 447–456
McAllister, C. D., Shah, N., Strickland, J. D. H. (1964). Marine phytoplankton photosynthesis as a function of light intensity: a comparison of methods. J. Fish. Res. Bd Can. 21: 159–181
Metealfe, L. D., Schmitz, A. A., Pelka, J. R. (1966). Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analyt. Chem. 38: 514–515
Morris, I., Skea, W. (1978). Products of photosynthesis in natural populations of marine phytoplankton from the Gulf of Maine. Mar. Biol. 47: 303–312
Morris, I., Smith, A. E., Glover, H. E. (1981). Products of photosynthesis in phytoplankton off the Orinoco River and in the Caribbean Sea. Limnol. Oceanogr. 26: 1034–1044
Myklestad, S., Haug, A. (1972). Production of carbohydrates by the marine diatom Chaetoceros affinis var. willei (Gran) Hustedt. I. Effect of the concentration of nutrients in the culture medium. J. exp. mar. Biol. Ecol. 9: 125–136
Peterson, B. J. (1980). Aquatic primary productivity and the14C-CO2 method: a history of the productivity problem. A. Rev. Ecol. Syst. 11: 359–385
Piorreck, M., Baasch, K. H., Pohl, P. (1984). Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry 23: 207–216
Pohl, P., Wagner, H. (1972). Control of fatty acid and lipid biosynthesis in Euglena gracilis by ammonia, light and DCMU. Z. Naturforsch. 27B: 53–61
Richardson, K., Samuelsson G., Hällgren, J. E. (1984). The relationship between photosynthesis measured by14C incorporation and by uptake of inorganic carbon in unicellular algae. J. exp. mar. Biol. Ecol. 81: 241–250
Roessler, P. G. (1988). Effects of silicon deficiency on lipid composition and metabolism in the diatom Cyclotella cryptica. J. Phycol. 24: 394–400
Ryther, J. H., Menzel D. W. (1965). Comparison of the14C-technique with direct measurement of photosynthetic carbon fixation. Limnol. Oceanogr. 10: 490–492
Shuter, B. (1979). A model of physiological adaptation in unicellular algae. J. theor. Biol. 78: 519–552
Smith, A. E., Morris, I. (1980). Synthesis of lipid during photosynthesis by phytoplankton of the Southern Ocean. Science, N. Y. 207: 197–199
Strickland, J. D. H., Parsons, T. R. (1972). A practical handbook of seawater analysis, 2nd edn., Part II, Inorganic micronutrients in sea water. Bull. Fish. Res. Bd Can. 167: 45–137
Suen, Y., Hubbard, J. S., Holzer, G., Tornabene, T. G. (1987). Total lipid production of the green alga Nannochloropsis sp. QII under different nitrogen regimes. J. Phycol. 23: 289–296
Williams, P. J. L. (1984). Bacterial production in the marine food chain: the emperor's new suit of clothes? In: Fasham, M. J. R. (ed.) Flows of energy and materials in marine ecosystems theory and practice. Plenum Press, New York, p. 271–299
Wood, B. J. B. (1974). Fatty acids and saponifiable lipids. In: Stewart, W. D. P. (ed.) Algal physiology and biochemistry. Blackwell Scientific Publications, Oxford, p. 236–265
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Communicated by M. Anraku, Suva
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Hama, J., Handa, N. Diel photosynthetic production of cellular organic matter in natural phytoplankton populations, measured with13C and gas chromatography/mass spectrometry. Mar. Biol. 112, 183–190 (1992). https://doi.org/10.1007/BF00702460
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DOI: https://doi.org/10.1007/BF00702460