Chemistry of Natural Compounds

, Volume 5, Issue 4, pp 244–246 | Cite as

The thin-layer chromatography of dinucleotides

  • K. A. Averburg
  • E. N. Morozova
  • N. S. Tikhomirova-Sidorova


1. The chromatographic behavior of the dinucleotides of an RNA hydrolysate in thin layers of ECTEOLA- and DEAE-cellulose and on a cellulose powder has been studied. The Rf values of the four dinucleotides ApCp, ApUp,GpCp andGpUp in various solvent systems have been determined. It has been shown that in a thin layer of DEAE cellulose the dinucleotide fraction of a ribonuclease hydrolysate is separated into the four individual components.

2. Conditions for the separation of authentic mixtures of dinucleotides and of mixtures of mono- and dinucleotides have been found.


Cellulose Dinucleotide HCOOH Ammonium Formate Chromatographic Behavior 


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Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • K. A. Averburg
    • 1
  • E. N. Morozova
    • 1
  • N. S. Tikhomirova-Sidorova
    • 1
  1. 1.Institute of High-Molecular-Weight Compounds AS USSRUSSR

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