Abstract
In order to study the characteristic of contraluminal transport of hydrophylic small fatty acids the in situ stopped flow microperfusion technique [12] has been applied. By measuring with 4 s contact time the decrease in the contraluminal concentration of the respective radiolabelled substances the concentration dependence of the influx into the cortical cells was tested. The 4 s decrease in contraluminal concentration of chloroacetate,l-lactate,d-lactate, 3-hydroxybutyrate and acetoacetate was between 26% and 31%. For each substance the percent decrease was the same, no matter whether it was offered in a concentration of 0.1 or 10 mmol/l. Contraluminal disappearance of 0.1 mmol/ll-lactate was not influenced by 5 mmol/l H2DIDS, probenecid, phloretin, mersalyl or cyanocinnamate, but it was significantly (37%) inhibited by 5-nitro-2-(phenyl-propyl-amino) benzoate, a blocker of the nonspecific anion channel. The percent decrease in propionate uptake was somewhat larger — between 36% and 39% — but again not different at 0.01, 0.1, 1.0 and 10 mmol/l. With pyruvate the contraluminal decrease was 20% at 0.1 mmol/l and 31% at 10 mmol/l. The percent disappearance of the aromatic pyrazinoate was 38% and 34% at 0.1 and 10 mmol/l and for nicotinate 42% and 22%, respectively. The disappearance of nicotinate (0.1 mmol/l) was significantly inhibited by 10 mmol/l pyrazinoate and paraaminohippurate (PAH). The data are in agreement with the hypothesis that the hydrophilic small fatty acids traverse the contraluminal cell side by simple diffusion, possibly via the unspecific anion channel [14], pyruvate via the dicarboxylic acid pathway in a cooperative manner and pyrazinoate, as well as nicotinate, via the PAH pathway.
Similar content being viewed by others
References
Barac-Nieto M (1985) Renal hydroxybutyrate and acetoacetate reabsorption and utilization in the rat. Am J Physiol 249:F40-F48
Barac-Nieto M, Murer H, Kinne R (1980) Lactate-sodium cotransport in rat renal brush border membranes. Am J Physiol 239:F496-F506
Barac-Nieto M, Murer H, Kinne R (1982) Asymmetry in the transport of lactate by basolateral and brush border membranes of rat kidney cortex. Pflügers Arch 392:366–371
Burckhardt G (1984) Sodium-dependent dicarboxylate transport in rat renal basolateral membrane vesicles. Pflügers Arch 401:254–261
Deutike B (1982) Monocarboxylate transport in erythrocytes. J Membr Biol 70:89–103
Deutike B, Rickert I, Beyer E (1978) Stereoselective SH dependent transfer of lactate in mammalian erythrocytes. Biochim Biophys Acta 507:137–155
Deutike B, Beyer E, Forst B (1982) Discrimination of three parallel pathways of lactate transport in the human erythrocyte membrane by inhibitors and kinetic properties. Biochim Biophys Acta 684:96–110
Diez F, Valdez JM, Vilet R, Garza R (1981) Lactate oxidation and sodium reabsorption by dog kidney in vivo. Am J Physiol 240:F343-F351
Dubinsky WP, Racker E (1978) The mechanism of lactate transport in human erythrocytes. J Membr Biol 44:25–36
Fafournoux P, Demigné C, Rémésy C (1985) Carrier-mediated uptake of lactate in rat hepatocytes. J Biol Chem 260:292–299
Foulkes EC, Paine CM (1961) The uptake of monocarboxylic acids by rat diaphragm. J Biol Chem 236:1019–1022
Fritzsch G, Haase W, Rumrich G, Fasold H, Ullrich KJ (1984) A stopped flow capillary perfusion method to evaluate contraluminal transport parameters of methylsuccinate from interstitium into renal proximal tubular cells. Pflügers Arch 400:250–256
Garcia ML, Benavides J, Valdivieso F (1980) Ketone body transport in renal brush border membrane vesicles. Biochim Biophys Acta 600:922–930
Gögelein H, Greger R (1986) A voltage dependent nonselective channel in the basolateral membrane of late proximal tubules of rabbit kidney. Pflügers Arch 407 (Suppl 2): in press
Guder WG, Pürschel S, Wirthensohn G (1985) Renal ketone body metabolism. In: Dzúrik R, Lichardus B, Guder W (eds) Kidney metabolism and function. Martinus Nijhoff Publ, Dortrecht Boston Lancaster, pp 93–102
Jørgensen KE, Sheikh MI (1984) Renal transport of monocarboxylic acids. Heterogeneity of lactate-transport systems along the proximal tubule. Biochem J 223:803–807
Krebs HA, Speake RN, Hems R (1965) Acceleration of renal gluconeogenesis by ketone bodies and fatty acids. Biochem J 94:712–720
Löw I, Friedrich T, Burckhardt G (1984) Properties of an anion exchanger in rat renal basolateral membrane vesicles. Am J Physiol 246:F334-F342
Lücke H, Stange G, Murer H (1979) Sulphate ion — sodium ion cotransport by brush border membrane vesicles from rat kidney cortex. Biochem J 182:223–229
Mann GE, Zlokovic BV, Yudilevich DL (1985) Evidence for a lactate transport system in the sarcolemmal membrane of the perfused rabbit heart: kinetics of unidirectional influx, carrier specificity and effects of glucagon. Biochim Biophys Acta 819:241–248
Mason MJ, Thomas RC (1985) Evidence for facilitated diffusion ofl-lactate across frog skeletal muscle membranes. J Physiol 361:23P
Mengual R, Leblanc G, Sudaka P (1983) The mechanism of Na+-l-lactate cotransport by brush-border membrane vesicles from horse kindey. J Biol Chem 258:15071–15078
Monson JP, Smith JA, Cohen RD, Iles RA (1982) Evidence for a lactate transporter in the plasma membrane of the rat hepatocyte. Clin Sci 62:411–420
Nord E, Wright SH, Kippen I, Wright EM (1982) Pathways for carboxylic acid transport by rabbit renal brush border membrane vesicles. Am J Physiol 234F456-F462.
Nord EP, Wright SH, Kippen I Wright EM (1983) Specificity of the Na+-dependent monocarboxylic acid transport pathway in rabbit renal brush border membranes. J Membr Biol 72:213–221
Regen DM, Tarpley HL (1980) Effects of pH on β-hydroxybutyrate exchange kinetics of rat erythrocytes. Biochim Biophys Acta 601:500–508
Rennie MJ, Watt PW (1985) Lactate transport studied in perfused rat skeletal muscle. J Physiol 365:96P
Schwab AJ, Bracht A, Scholz R (1979) Transport ofd-lactate in perfused rat liver. Eur J Biochem 102:537–547
Seo Y (1984) Effects of extracellular pH on lactate efflux from frog sartorius muscle. Am J Physiol 247:C175-C181
Sheridan E, Rumrich G, Ullrich KJ (1983) Reabsorption of dicarboxylic acids from the proximal convolution of rat kidney. Pflügers Arch 399:18–28
Spencer TL, Lehninger AL (1976)l-lactate transport in Ehrlich ascites-tumor cells. Biochem J 154:405–414
Ullrich KJ, Papavassiliou F (1985) Contraluminal transport of hexoses in the proximal convolution of the rat kidney in situ. Pflügers Arch 404:150–156
Ullrich KJ, Rumrich G, Klöss S (1980) Active sulfate reabsorption in the proximal convolution of the rat kidney: specificity, Na+ and HCO −3 dependence. Pflügers Arch 383:159–163
Ullrich KJ, Rumrich G, Klöss S (1982) Reabsorption of monocarboxylic acids in the proximal tubule of the rat kidney. I, II and III. Pflügers Arch 395:212–231
Ullrich KJ, Fasold H, Rumrich G, Klöss S (1984) Secretion and contraluminal uptake of dicarboxylic acids in the proximal convolution of rat kidney. Pflügers Arch 400:241–249
Ullrich KJ, Rumrich G, Klöss S (1984), Contraluminal sulfate transport in the proximal tubule of the rat kidney. Pflügers Arch 402:264–271
Ullrich KJ, Papavassiliou F, Rumrich G, Fritzsch G (1985) Contraluminal phosphate transport in the proximal tubule of the rat kidney. Pflügers Arch 405:S106-S109
Wangemann P, Wittner M, Di Stefano A, Englert HC, Lang HJ, Schlatter E, Greger R. Cl−-channel blockers in the thick ascending limb of the loop of Henle. Structure activity relationship. Pflügers Arch 407 (Suppl 2): in press
Wright EM (1985) Transport of carboxylic acids by renal membrane vesicles. Ann Rev Physiol 47:127–141
Wright SH, Kippen I, Klinenberg JR,Wright EM (1980) Specificity of the transport system for tricarboxylic acid cycle intermediates in renal brush borders. J Membr Biol 57:73–82
Wright SH, Hirayama B, Kaunitz, JD, Kippen I, Wright EM (1983) Kinetics of sodium succinate cotransport across renal brush border membranes. J Biol Chem 258:5456–5462
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ullrich, K.J., Papavassiliou, F. Contraluminal transport of small aliphatic carboxylates in the proximal tubule of the rat kidney in situ. Pflugers Arch. 407, 488–492 (1986). https://doi.org/10.1007/BF00657505
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00657505