Abstract
We have examined a serum-deprived culture system in order to verify that it is suitable for the study of burst forming unit erythroid (BFU-E) progenitor cells from premature neonates. Optimum growth of BFU-E from premature neonates was observed with each media constituent using the same concentration as that previously described for adult subjects. Growth of immature BFU-E from premature neonates were highly dependant upon a source of Burst Promoting Activity and mature BFU-E derived colonies emerged at day 12 compared to day 14 in adults. Our preliminary results with the validated medium suggest that premature infants have increased peripheral blood concentrations of BFU-E compared to healthy adult controls.
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Abbreviations
- Ad:
-
Adherent cells
- BPA:
-
Burst promoting activity
- BFU-E:
-
Burst forming unit erythroid
- Epo:
-
Erythropoietin
- IL3:
-
Interleukin-3
- LDC:
-
Low density (<1.077 g ml1) peripheral blood mononuclear cells
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Westwood, N.B., Barrett Emmerson, A.J. Erythropoietic progenitor cells from premature neonates studied using a validated serum-deprived medium. Cytotechnology 6, 241–246 (1991). https://doi.org/10.1007/BF00624763
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DOI: https://doi.org/10.1007/BF00624763